Proper Citation: EuMMCR (RRID:SCR_001506)
Description: Embryonic stem cell distribution unit that distributes material arising within European Conditional Mouse Mutagenesis Program consortium, currently targeting vectors and ES cells. Upon user request EUCOMM grow targeting vectors from glycerol stocks and prepare vector DNA. Identity of vector is verified by restriction mapping. Upon user request EUCOMM thaw, expand and re-freeze several aliquots of desired ES cell clone. Standard controls include PCR based assay. Upon additional request EuMMCR unit develops genotyping PCR, which can be used to genotype chimeric mice that may be generated using those ES cell clones.
Abbreviations: EuMMCR
Synonyms: EuMMCR - European Mouse Mutant Cell Repository, European Mouse Mutant Cell Repository
Resource Type: cell repository, biomaterial supply resource, material resource
Keywords: embryonic stem cell, vector, experimental protocol, male, dna, genotype
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