University of Pittsburgh Genome Editing, Transgenic and Virus Core Facility (RRID:SCR_025151)Copy Citation Copied
Proper Citation: University of Pittsburgh Genome Editing, Transgenic and Virus Core Facility (RRID:SCR_025151)
Description: Core provides technical expertise, advice, and training and is comprised of three functional sections: Molecular cloning (plasmid, virus, CRISPR/Cas9); Viral vectors (lentivirus, adenovirus, adeno-associated virus); Animal services (transgenic, knockout, cryopreservation, re-derivation, and more). Services offered include Assistance for generating recombinant plasmids; Design and implementation of CRISPR/Cas9 technology for in vivo and in vitro work; Mouse and rat transgenesis,including transgenic and knockout/knockdown techniques using pronuclear injection, ES cell injection/chimera, and CRISPR/Cas9-mediated genome editing; Production of lentivirus, adenovirus, and adeno-associated virus vectors; Additional animal testing and procedures: teratoma and chimera testing to validate putative pluripotent stem cell lines, cryopreserve or re-derive of valuable transgenic lines (sperm or embryos).
Abbreviations: GETV
Synonyms: , Genome Editing, Transgenic, Transgenic and Virus (GETV) Core, and Virus (GETV) Core Facility, MWRI Genome Editing
Resource Type: service resource, access service resource, core facility
Keywords: ABRF, Molecular cloning, Viral vectors, Animal services, CRISPR/Cas9 technology, mouse and rat transgenesis, lentivirus, adenovirus, and adeno associated virus vectors production,
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