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University of California at San Francisco Division of Experimental Medicine Flow Core Facility (RRID:SCR_017903)
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Resource Information

URL: http://coreimmunology.ucsf.edu/flow-cytometry

Proper Citation: University of California at San Francisco Division of Experimental Medicine Flow Core Facility (RRID:SCR_017903)

Description: Flow cytometry facility offering training and services including:Access to two, 17-color BD LSR II analytical instruments with High Throughput Sampler (HTS) module,Configurations:LSRII 1,LSRII 2;Help with Flow Cytometry Panel Design;Fluorofinder (access our cytometers under CFAR Immunology Core);BD Panel designer;SFGH LSRII Flow Core Protocols;LSRII Startup and Shutdown;How to run the CST calibration assay;Access to a 17-color BD FACSAria II for fluorescence-activated cell sorting (FACS);4-way tube sorting;96 well plate sorting;Index sorting;SFGH ARIA Flow Core Protocols and configuration;ARIA Startup;Determining Drop Delay;Side Stream Set Up;Clog Procedure;ARIA Shutdown Protocol;ARIA Configuration;DNA analysis with standard dyes;Analysis of CFP, GFP, YFP, mRFP, mTomato, and mCherry gene expression proteins;Calcium flux measurements using Indo-1;Training of users on the operation of instruments and experimental design through the CIL Flow Cytometry Course;Maintaining and Upgrading Instruments;Research Support Services (study design, assay selection, grant and paper writing support).

Synonyms: Flow Cytometry Core

Resource Type: service resource, access service resource, core facility

Keywords: Flow, cytometry, support, training, instrumentation, study, design, assay, grant, paper, service, core

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