Nebraska University Medical Center Mouse Genome Engineering Core Facility (RRID:SCR_017755)Copy Citation Copied
URL: https://www.unmc.edu/vcr/cores/vcr-cores/mgec/index.html
Proper Citation: Nebraska University Medical Center Mouse Genome Engineering Core Facility (RRID:SCR_017755)
Description: Core Facility provides expertise and advice for experimental design of transgenic or gene knockout experiments, including DNA construct production and genotyping assays, makes reagents available for generation of transgene or gene targeting constructs, and performs all experimental aspects, which include pronuclear injection of transgene constructs, generation of recombinant mouse ES cells, blastocyst injection, and embryo transfer surgeries, for generation or rederivation of genetically manipulated mouse strains. Transgenic founder mice or chimeric animals with targeted alleles are then transferred to individual investigator for analysis.
Synonyms: UNMC Mouse Genome Engineering Core Facility
Resource Type: access service resource, core facility, service resource
Keywords: Transgenic, gene, knockout, mice, experiment, DNA, construction, production, genotyping, assay, construct, injection, recombinant, ES cell, blastocyst, embryo, surgery, chimeric, animal, analysis, service, core
Availability: Open
Resource Name: Nebraska University Medical Center Mouse Genome Engineering Core Facility
Resource ID: SCR_017755
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Source: SciCrunch Registry (RRID:SCR_005400)
Description: Interactive portal for finding and submitting biomedical resources. Resources within SciCrunch have assigned RRIDs which are used to cite resources in scientific manuscripts. SciCrunch Registry, formerly NIF Registry, provides resources catalog. Allows to add new resources. Allows edit existing resources after registration. Curators are tasked with identifying and registering resources, examining data, writing configuration files to index and display data and keeping contents current.
URL: https://rrid.site/rin/sources/SCR_005400