Nebraska University Medical Center Mouse Genome Engineering Core Facility (RRID:SCR_017755)Copy Citation Copied
URL: https://www.unmc.edu/vcr/cores/vcr-cores/mgec/index.html
Proper Citation: Nebraska University Medical Center Mouse Genome Engineering Core Facility (RRID:SCR_017755)
Description: Core Facility provides expertise and advice for experimental design of transgenic or gene knockout experiments, including DNA construct production and genotyping assays, makes reagents available for generation of transgene or gene targeting constructs, and performs all experimental aspects, which include pronuclear injection of transgene constructs, generation of recombinant mouse ES cells, blastocyst injection, and embryo transfer surgeries, for generation or rederivation of genetically manipulated mouse strains. Transgenic founder mice or chimeric animals with targeted alleles are then transferred to individual investigator for analysis.
Synonyms: UNMC Mouse Genome Engineering Core Facility
Resource Type: service resource, access service resource, core facility
Keywords: Transgenic, gene, knockout, mice, experiment, DNA, construction, production, genotyping, assay, construct, injection, recombinant, ES cell, blastocyst, embryo, surgery, chimeric, animal, analysis, service, core
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