URL: http://g2im.u-clermont1.fr/hispod/index.php
Proper Citation: HiSpOD (RRID:SCR_014403)
Description: A software dedicated to microbial ecology and environmental studies which uses individual nucleic sequences or consensus sequences produced by multiple alignments to design degenerate probes in order to target gene families covering many sequence variants. Probe specificity is assessed by similarity search against an exhaustive formatted database dedicated to microbial ecology containing coding sequences (CDS) and their putative 5-prime and 3-prime unstranslated regions (UTR). Potential cross-hybridizations identified by the program are clustered and results are organized in order to simplify user interpretation.
Synonyms: High Specific Oligonucleotide Design (HiSpOD), High Specific Oligonucleotide Design
Resource Type: software application, software resource, standalone software
Defining Citation: PMID:21216777, DOI:10.1093/bioinformatics/btq712
Keywords: probe design, microbial ecology, environmental studies, nucleic sequence, consensus sequence, sequence variant, probe specificity, utr, cds
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