URL: https://web.expasy.org/cellosaurus/CVCL_N806
Proper Citation: (RRID:CVCL_N806)
Description: Cell line WI-38/hTERT/GFP-RAF-ER is a Telomerase immortalized cell line with a species of origin Homo sapiens (Human)
Sex: Female
Comments: Omics: H3K4me3 ChIP-seq epigenome analysis., Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP)., Transfected with: HGNC; 11730; TERT., Transfected with: HGNC; 3467; ESR1 (hormone-binding domain)., Transfected with: HGNC; 9829; RAF1 (catalytic domain with D-340 and D-341)., Senescence: Cells can be senesced after exposure for 72 hours with 4-hydroxy-tamoxifen., Characteristics: Transfected with a construct that is composed of EGFP at the N-terminus, the catalytic domain of RAF1 (with 340-DD-341) in the middle and the hormone-binding domain of ESR1 at the C-terminus. Protein kinase activity is activated by estrogen or analogs such as 4-hydroxy-tamoxifen., Population: Caucasian; Swedish., Part of: ENCODE project common cell types; tier 3.
Category: Telomerase immortalized cell line
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Source: Cellosaurus