URL: https://web.expasy.org/cellosaurus/CVCL_DA04
Proper Citation: (RRID:CVCL_DA04)
Description: Cell line Tau RD P301S FRET Biosensor is a Transformed cell line with a species of origin Homo sapiens (Human)
Sex: Female
Defining Citation: PMID:25261551, PMID:37553663
Comments: Transfected with: UniProtKB; P00552; Transposon Tn5 neo., Transfected with: UniProtKB; P42212; YFP (modified GFP at G-65, L-68, A-72, Y-203 and L-231)., Transfected with: UniProtKB; Q66PV6; CFP., Characteristics: Tau seeds introduced into the culture media of this cell line can nucleate the aggregation of the reporter proteins. This aggregation produces a FRET signal which can be measured via microscopy, microplate readers or flow cytometry (ATCC=CRL-3275)., Characteristics: Transduced with 2 separate lentivirus constructs encoding the tau repeat domain (RD) with the disease-associated p.Pro301Ser (p.Pro636Ser) mutation fused to either CFP or YFP (ATCC=CRL-3275).
Category: Transformed cell line
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Source: Cellosaurus
