URL: https://web.expasy.org/cellosaurus/CVCL_5440
Proper Citation: (ATCC Cat# CRL-2593, RRID:CVCL_5440)
Description: Cell line MC3T3-E1 Subclone 4 is a Spontaneously immortalized cell line with a species of origin Mus musculus
Sex: Sex unspecified
Defining Citation: PMID:10352097, PMID:23430347
Comments: Breed/subspecies: C57BL/6., Derived from sampling site: Bone; calvaria. Cell type=Osteoblast., Miscellaneous: STR profile from personal communication of Almeida J.L.; PubMed=23430347 has a different value for STR 6-4 (17) than that submitted (18) due to a change in the marker motif.., Doubling time: 51.6 hours (CelloPub=CLPUB00625); ~38 hours (ATCC)., Characteristics: Exhibits high levels of osteoblast differentiation after growth in ascorbic acid and 3 to 4 mM inorganic phosphate. Forms a well mineralized extracellular matrix (ECM) after 10 days (ATCC).
Category: Spontaneously immortalized cell line
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Source: Cellosaurus
