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QGP-1

RRID:CVCL_3143

Organism

Homo sapiens

Comments

Part of: Cancer Cell Line Encyclopedia (CCLE) project. Part of: COSMIC cell lines project. Part of: NCI RAS program mutant KRAS cell line panel. Doubling time: ~3.5 days (PubMed=7227711); 61 hours (PubMed=25984343); 1.6 +- 0.3 days (PubMed=29330294); ~42 hours (lot 04112012), ~2 days (lot 02162009), 40-50 hours (lot 04152003), ~74 hours (lot 05312016) (JCRB). Microsatellite instability: Stable (MSS) (Sanger). Sequence variation: Heterozygous for KRAS p.Gly12Val (c.35G>T) (PubMed=21607521; CCLE; Cosmic-CLP). Sequence variation: Heterozygous for TP53 p.Pro98fs*25 (c.293delC) (CCLE; Cosmic-CLP). Omics: Deep exome analysis. Omics: Deep RNAseq analysis. Omics: DNA methylation analysis. Omics: shRNA library screening. Omics: SNP array analysis. Omics: Transcriptome analysis. Genome ancestry: African=0%; Native American=0%; East Asian, North=82.5%; East Asian, South=17.02%; South Asian=0.48%; European, North=0%; European, South=0% (PubMed=30894373). Misspelling: QGP; In CGH-DB 9375-4 and GEO GSM600373. Misspelling: QCP-1; In DOI=10.5772/30884. DT Created: 04-04-12; Last updated: 06-09-19; Version: 27

Proper Citation

JCRB Cat# JCRB0183, RRID:CVCL_3143

Category

Cancer cell line DT Created: 04-04-12; Last updated: 06-09-19; Version: 27

Sex

DT Created: 04-04-12; Last updated: 06-09-19; Version: 27

Synonyms

QGP 1, QGP1 DT Created: 04-04-12, Last updated: 06-09-19, Version: 27

Vendor

JCRB

Cat Num

JCRB0183

Cross References

BTO; BTO:0005500 EFO; EFO_0006741 ArrayExpress; E-MTAB-2706 ArrayExpress; E-MTAB-2770 ArrayExpress; E-MTAB-3610 BioSample; SAMN03472832 BioSample; SAMN03472892 BioSample; SAMN10988306 CCLE; QGP1_PANCREAS Cell_Model_Passport; SIDM00360 CGH-DB; 9375-4 Cosmic; 736280 Cosmic; 922260 Cosmic; 933517 Cosmic; 1620106 Cosmic; 1879412 Cosmic; 2433551 Cosmic; 2530677 Cosmic-CLP; 1298534 DepMap; ACH-000347 GDSC; 1298534 GEO; GSM600373 GEO; GSM830112 GEO; GSM827299 GEO; GSM887522 GEO; GSM888604 GEO; GSM1374837 GEO; GSM1670355 IARC_TP53; 30149 JCRB; JCRB0183 LiGeA; CCLE_506 LINCS_LDP; LCL-1754 PharmacoDB; QGP1_1280_2019 Wikidata; Q54948822 DT Created: 04-04-12; Last updated: 06-09-19; Version: 27

Hierarchy

DT Created: 04-04-12; Last updated: 06-09-19; Version: 27

Originate from Same Individual

DT Created: 04-04-12; Last updated: 06-09-19; Version: 27

Publications that use this research resource

Heterodimerization of two pore domain K+ channel TASK1 and TALK2 in living heterologous expression systems.

  • Suzuki Y
  • PLoS ONE
  • 2017 Oct 19

Literature context:


Abstract:

Two-pore-domain K+ (K2P) channels sense a wide variety of stimuli such as mechanical stress, inhalational anesthetics, and changes in extracellular pH or temperature. The K2P channel activity forms a background K+ current and, thereby, contributes to resting membrane potentials. Six subfamilies including fifteen subtypes of K2P channels have been identified. Each K2P channel molecule with two pores consists of a homodimer of each subtype. In addition, a few heterodimers mainly within the same subfamilies have been found recently. In the present study, the possibility of heterodimerization between TASK1 (TWIK-Related Acid-Sensitive K+ channel) and TALK2 (TWIK-Related Alkaline pH-Activated K+ channel) was examined. These channels belong to separate subfamilies and show extremely different channel properties. Surprisingly, single molecular imaging analyses in this study using a total internal reflection microscope suggested the heterodimerization of TASK1 and TALK2 in a pancreatic cell line, QGP-1. This heterodimer was also detected using a bimolecular fluorescence complementation assay in a HEK293 heterologous expression system. Fluorescence resonance energy transfer analyses showed that the affinity between TASK1 and TALK2 appeared to be close to those of homodimers. Whole-cell patch-clamp recordings revealed that TASK1 currents in HEK293 cells were significantly attenuated by co-expression of a dominant-negative form of TALK2 in comparison with that of wild-type TALK2. The sensitivities of TASK1-TALK2 tandem constructs to extracellular pH and halothane were characterized as a unique hybrid of TASK1 and TALK2. These results suggested that heterodimerization of TASK1 and TALK2 provides cells with the ability to make multiple responses to a variety of physiological and pharmacological stimuli.

Funding information:
  • NIMH NIH HHS - R01 MH101130(United States)