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M059J

RRID:CVCL_0400

Organism

Homo sapiens

Comments

Part of: Cancer Cell Line Encyclopedia (CCLE) project. Part of: COSMIC cell lines project. Part of: ENCODE project common cell types; tier 3. Characteristics: Radiosensitive (PubMed=7494872). Characteristics: Has a near pentaploid phenotype. Microsatellite instability: Stable (MSS) (Sanger). Sequence variation: Heterozygous for PRKDC p.Thr1351Profs*8 (c.4051delA) (PubMed=11418067; CCLE). Sequence variation: Homozygous for PTEN p.Pro204fs*17 (c.610delC) (ATCC; CCLE; Cosmic-CLP). Sequence variation: Homozygous for TP53 p.Glu286lys (c.856G>A) (PubMed=11023613; CCLE; Cosmic-CLP). Omics: Deep exome analysis. Omics: Deep RNAseq analysis. Omics: DNA methylation analysis. Omics: SNP array analysis. Omics: Transcriptome analysis. Genome ancestry: African=1.01%; Native American=0%; East Asian, North=2.75%; East Asian, South=0%; South Asian=0%; European, North=67.68%; European, South=28.55% (PubMed=30894373). Caution: Was incorrectly stated to have deleterious mutations in ATM in PubMed=12105990. DT Created: 04-04-12; Last updated: 06-09-19; Version: 26

Proper Citation

BCRJ Cat# 0151, RRID:CVCL_0400

Category

Cancer cell line DT Created: 04-04-12; Last updated: 06-09-19; Version: 26

Sex

DT Created: 04-04-12; Last updated: 06-09-19; Version: 26

Synonyms

Mo 59J, MO59J DT Created: 04-04-12, Last updated: 06-09-19, Version: 26

Vendor

BCRJ

Cat Num

0151

Cross References

CLO; CLO_0007445 EFO; EFO_0005697 MCCL; MCC:0000294 ArrayExpress; E-MTAB-2706 ArrayExpress; E-MTAB-3610 ATCC; CRL-2366 BCRC; 60382 BCRJ; 0151 BioSample; SAMN03471832 BioSample; SAMN03473044 CCLE; M059J_CENTRAL_NERVOUS_SYSTEM Cell_Model_Passport; SIDM00659 ChEMBL-Cells; CHEMBL3308113 ChEMBL-Targets; CHEMBL1075494 Cosmic; 949094 Cosmic; 1746953 Cosmic; 2367537 Cosmic-CLP; 949094 DepMap; ACH-001118 ENCODE; ENCBS062ILC ENCODE; ENCBS235AAA ENCODE; ENCBS304XWS ENCODE; ENCBS370AFW GDSC; 949094 GEO; GSM326251 GEO; GSM1374640 GEO; GSM1670070 IARC_TP53; 13244 LINCS_LDP; LCL-1356 PharmacoDB; M059J_877_2019 Wikidata; Q54903415 DT Created: 04-04-12; Last updated: 06-09-19; Version: 26

Hierarchy

DT Created: 04-04-12; Last updated: 06-09-19; Version: 26

Originate from Same Individual

DT Created: 04-04-12; Last updated: 06-09-19; Version: 26

Publications that use this research resource

The Histone Chaperones ASF1 and CAF-1 Promote MMS22L-TONSL-Mediated Rad51 Loading onto ssDNA during Homologous Recombination in Human Cells.

  • Huang TH
  • Mol. Cell
  • 2018 Mar 1

Literature context:


Abstract:

The access-repair-restore model for the role of chromatin in DNA repair infers that chromatin is a mere obstacle to DNA repair. However, here we show that blocking chromatin assembly, via knockdown of the histone chaperones ASF1 or CAF-1 or a mutation that prevents ASF1A binding to histones, hinders Rad51 loading onto ssDNA during homologous recombination. This is a consequence of reduced recruitment of the Rad51 loader MMS22L-TONSL to ssDNA, resulting in persistent RPA foci, extensive DNA end resection, persistent activation of the ATR-Chk1 pathway, and cell cycle arrest. In agreement, histones occupy ssDNA during DNA repair in yeast. We also uncovered DNA-PKcs-dependent DNA damage-induced ASF1A phosphorylation, which enhances chromatin assembly, promoting MMS22L-TONSL recruitment and, hence, Rad51 loading. We propose that transient assembly of newly synthesized histones onto ssDNA serves to recruit MMS22L-TONSL to efficiently form the Rad51 nucleofilament for strand invasion, suggesting an active role of chromatin assembly in homologous recombination.

Funding information:
  • Intramural NIH HHS - (United States)
  • NCI NIH HHS - R01 CA095641()

MALDI imaging detects endogenous digoxin in glioblastoma cells infected by Zika virus-Would it be the oncolytic key?

  • Lima EO
  • J Mass Spectrom
  • 2017 Dec 30

Literature context:


Abstract:

Funding information:
  • NIMH NIH HHS - R01 MH066912(United States)