URL: http://www.addgene.org/79888
Proper Citation: RRID:Addgene_79888
Bacterial Resistance: Ampicillin
Defining Citation: PMID:26456817
Vector Backbone Description: Vector Backbone:pX330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: This plasmid is derived from eSpCas9(1.1) (Plasmid #71814) from Feng Zhang's lab. The 3x FLAG sequence was removed to prevent interference of the tag in western blot and immunoprecipitation applications. The gRNA sequence is derived from gRNA_AAVS1-T2 (Plasmid #41818) from George Church's lab.
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Source: Addgene
