URL: http://www.addgene.org/64218
Proper Citation: RRID:Addgene_64218
Insert Name: Cas9
Organism: Streptococcus pyogenes
Bacterial Resistance: Ampicillin
Defining Citation: PMID:25803306
Vector Backbone Description: Backbone Marker:Kuhn lab (Addgene plasmid # 64323); Vector Backbone:pU6-(BbsI)_CBh-Cas9-T2A-BFP; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: Please note that due to the presence of BbsI sites in the Ad4 coding regions it is NOT possible to directly clone new sgRNA sequences into the BbsI sites downstream of the U6 promoter. Therefore, precloned U6-sgRNA cassettes, isolated as 1.7 kb PvuI-XbaI fragments from pU6-(BbsI)_CBh-Cas9-T2A-BFP (plasmid# 64323) or pU6-(BbsI)_CBh-Cas9-T2A-mCherry (plasmid# 64324) of this deposit, or any other plasmid derived from pX330 (plasmid# 42230), must be ligated into the PvuI-XbaI digested backbone of this Ad4 containing plasmid.
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No rating or validation information has been found for pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
