URL: http://www.addgene.org/48250
Proper Citation: RRID:Addgene_48250
Insert Name: SypHer
Organism: Synthetic
Bacterial Resistance: Kanamycin
Defining Citation: PMID:21224385
Vector Backbone Description: Backbone Marker:Evrogen; Vector Backbone:pHyper-cyto; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: pHyper was cloned by replacing EGFP with the Hyper sequence in the pEGFP-C1 vector (Clontech). To make pSypHer, the C199S mutation was made with the QuikChange II site-directed mutagenesis kit using the following oligos: 5′-agatggtcactctttgcgcgat-3′ (forward) 5′-atcgcgcaaagagtgaccatct-3′ (reverse)
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org