URL: http://www.addgene.org/48250
Proper Citation: RRID:Addgene_48250
Insert Name: SypHer
Organism: Synthetic
Bacterial Resistance: Kanamycin
Defining Citation: PMID:21224385
Vector Backbone Description: Backbone Marker:Evrogen; Vector Backbone:pHyper-cyto; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: pHyper was cloned by replacing EGFP with the Hyper sequence in the pEGFP-C1 vector (Clontech). To make pSypHer, the C199S mutation was made with the QuikChange II site-directed mutagenesis kit using the following oligos: 5′-agatggtcactctttgcgcgat-3′ (forward) 5′-atcgcgcaaagagtgaccatct-3′ (reverse)
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Source: Addgene
