URL: http://www.addgene.org/23889
Proper Citation: RRID:Addgene_23889
Insert Name: MET
Organism: Homo sapiens
Bacterial Resistance: Spectinomycin
Defining Citation: PMID:21107320
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Comments: Please note that though the depositor's sequence suggests that this plasmid has MET transcript variant 1, a requesting scientist who sequenced the entire insert determined that this plasmid actually contains transcript variant 2 (NM_000245). The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections. A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
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Source: Addgene
