URL: http://www.addgene.org/16595
Proper Citation: RRID:Addgene_16595
Insert Name: c-myc promoter (TBE 1/2 wt)
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:9727977
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Comments: To construct pTBE1/2 plasmid, we used polymerase chain reaction (PCR) primers (5'-CTAGCTAGCCTAGCACCTTTGATTTCTCCC-3' and 5'-CGTGATATCCGCTTTGATCAAGAGTCCCAG-3') to amplify nt -576 to -1162 of the c-MYC promoter region. The PCR product was cloned into pBV-Luc. TBE1 (Tcf-4 binding elements) wt sequence cctttgatt; TBE2 wt sequence gctttgatc. See Author's Map and article for more information.
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