URL: http://www.addgene.org/16451
Proper Citation: RRID:Addgene_16451
Insert Name: WAF1 promoter
Organism: Homo sapiens
Bacterial Resistance: Chloramphenicol
Defining Citation: PMID:8242752
Vector Backbone Description: Backbone Size:5600; Vector Backbone:pBluescript II KS (+) + luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Chloramphenicol
Comments: The 2.4kb WAF1 promoter region was obtained by PCR amplification using a P1-WAF1 subclone as template and the primers 5’-CCACAAGCTTCTGACTTCGGCAG-3’and 5’CCCAGGAACAAGCTTGGGCAGCAG-3’. This PCR fragment was cloned into the HindIII site of pBluescript KS(+) (Stratagene) to yield plasmid pWWP. WWP-Luc was cloned by inserting the 2.6kb BamHl luciferase cassette from PG13-Luc into the Xhol sites of pWWP. Please note that there are a few discrepancies between Addgene's quality control sequence and the top GenBank hit for the promoter sequence. The plasmid should function as reported.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org