URL: http://www.addgene.org/16451
Proper Citation: RRID:Addgene_16451
Insert Name: WAF1 promoter
Organism: Homo sapiens
Bacterial Resistance: Chloramphenicol
Defining Citation: PMID:8242752
Vector Backbone Description: Backbone Size:5600; Vector Backbone:pBluescript II KS (+) + luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Chloramphenicol
Comments: The 2.4kb WAF1 promoter region was obtained by PCR amplification using a P1-WAF1 subclone as template and the primers 5’-CCACAAGCTTCTGACTTCGGCAG-3’and 5’CCCAGGAACAAGCTTGGGCAGCAG-3’. This PCR fragment was cloned into the HindIII site of pBluescript KS(+) (Stratagene) to yield plasmid pWWP. WWP-Luc was cloned by inserting the 2.6kb BamHl luciferase cassette from PG13-Luc into the Xhol sites of pWWP. Please note that there are a few discrepancies between Addgene's quality control sequence and the top GenBank hit for the promoter sequence. The plasmid should function as reported.
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Source: Addgene
