URL: http://www.addgene.org/163643
Proper Citation: RRID:Addgene_163643
Insert Name: pU6-CHYRON17 hgRNA
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:33753928
Vector Backbone Description: Backbone Marker:Phil Sharp and Feng Zhang, Addgene Plasmid #63592; Backbone Size:8251; Vector Backbone:lenti, 3rd generation; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: The mutations that make the sgRNA scaffold self-targeting or homing are described in Perli et al., 2016, doi: 10.1126/science.aag0511 and Kalhor et al., 2017, doi: 10.1038/nmeth.4108. This is a 3rd generation lentiviral transfer plasmid. Please visit https://doi.org/10.1101/639120 for bioRxiv preprint.
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Source: Addgene