URL: http://www.addgene.org/12341
Proper Citation: RRID:Addgene_12341
Insert Name: BRCA1
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:9207057
Vector Backbone Description: Backbone Marker:Verma Lab; Backbone Size:0; Vector Backbone:pCLMFG; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: Full-length BRCA1 cDNA was lifted from this construct as a NcoI (partial digest)-XbaI/blunted fragment and inserted into NcoI (partial digest)-cut and BamHI-cut/blunted pCL-MFG retroviral vector. Addgene's quality control sequence analysis identified a sequence discrepancy relative to Genbank reference sequence NM_007294, resulting in a P871L amino acid substitution in the encoded insert.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org