URL: http://www.addgene.org/12341
Proper Citation: RRID:Addgene_12341
Insert Name: BRCA1
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:9207057
Vector Backbone Description: Backbone Marker:Verma Lab; Backbone Size:0; Vector Backbone:pCLMFG; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: Full-length BRCA1 cDNA was lifted from this construct as a NcoI (partial digest)-XbaI/blunted fragment and inserted into NcoI (partial digest)-cut and BamHI-cut/blunted pCL-MFG retroviral vector. Addgene's quality control sequence analysis identified a sequence discrepancy relative to Genbank reference sequence NM_007294, resulting in a P871L amino acid substitution in the encoded insert.
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No rating or validation information has been found for pCL-MFG-BRCA1.
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Source: Addgene
