URL: http://www.addgene.org/104991
Proper Citation: RRID:Addgene_104991
Bacterial Resistance: Ampicillin
Defining Citation: PMID:30894629
Vector Backbone Description: Backbone Marker:Brett Stringer from Feng Zhang plasmid lentiCRISPR v2 (Addgene plasmid #52961); Backbone Size:11022; Vector Backbone:lentiCRISPRv2 hygro (Addgene #98291); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Comments: This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with a customizable sgRNA from a U6 promoter and hygromycin resistance gene from an EF-1a core promoter. This plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9 puro, lentiCas9 neo or lentiCas9 blast or with cell lines expressing Cas9. After cloning a double-stranded oligonucleotide specifying a gRNA between the two BsmBI restriction sites, use hU6-F (5'-GAGGGCCTATTTCCCATGATT-3') or LKO.1 5’(5'- GACTATCATATGCTTACCGT-3') to sequence the chimeric RNA element. Instruction for gRNA double-stranded oligonucleotide design and cloning can be found in the Zhang lab protocol, https://media.addgene.org/data/plasmids/52/52961/52961-attachment_B3xTwla0bkYD.pdf
Expand AllWe found {{ ctrl2.mentions.total_count }} mentions in open access literature.
We have not found any literature mentions for this resource.
We are searching literature mentions for this resource.
Most recent articles:
{{ mention._source.dc.creators[0].familyName }} {{ mention._source.dc.creators[0].initials }}, et al. ({{ mention._source.dc.publicationYear }}) {{ mention._source.dc.title }} {{ mention._source.dc.publishers[0].name }}, {{ mention._source.dc.publishers[0].volume }}({{ mention._source.dc.publishers[0].issue }}), {{ mention._source.dc.publishers[0].pagination }}. (PMID:{{ mention._id.replace('PMID:', '') }})
Check
for all resource mentions.
A list of researchers who have used the resource and an author search tool
Find mentions based on location
{{ ctrl2.mentions.errors.location }}
A list of researchers who have used the resource and an author search tool. This is available for resources that have literature mentions.
No rating or validation information has been found for lenti-sgRNA hygro.
No alerts have been found for lenti-sgRNA hygro.
Source: Addgene
