URL: http://antibodyregistry.org/AB_887871
Proper Citation: (Synaptic Systems Cat# 131 002, RRID:AB_887871)
Target Antigen: VGAT (cytoplasmic domain)
Host Organism: rabbit
Clonality: polyclonal
Comments: Applications: WB,IP,ICC,IHC,IHC-P. KO validated
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No rating or validation information has been found for Anti-VGAT.
Possibly Non-Specific
Extracted Antibody Information: "Briefly, slices were incubated in blocking solution containing 3% bovine serum albumin, 10% goat serum and 0.3% Triton in PBS (pH 7.4) for 1 h at room temperature. Primary antibodies rabbit anti-VIAAT (1:500; RRID: AB_887871;"
Extracted Specificity Statement: "Subsequently, slices were again rinsed in PBS, mounted on slides, air dried and cover slipped. Although, specificity of the anti-VIAAT antibody was previously shown in VIAAT knockout animals by the manufacturer (https://www.sysy.com/products/vgat/facts-131002.php), control experiments were performed by blocking the primary antibody with the corresponding control peptide (Catalog No. 131-OP, Synaptic Systems)."
Data was mined by Antibody Watch (https://arxiv.org/pdf/2008.01937.pdf), from PMID:29073893
Possibly Non-Specific
Extracted Antibody Information: "Primary antibodies rabbit anti-VIAAT (1:500; RRID: AB_887871;"
Extracted Specificity Statement: "Subsequently, slices were again rinsed in PBS, mounted on slides, air dried and cover slipped. Although, specificity of the anti-VIAAT antibody was previously shown in VIAAT knockout animals by the manufacturer (https://www.sysy.com/products/vgat/facts-131002.php), control experiments were performed by blocking the primary antibody with the corresponding control peptide (Catalog No. 131-OP, Synaptic Systems)."
Data was mined by Antibody Watch (https://arxiv.org/pdf/2008.01937.pdf), from PMID:29073893
Source: Antibody Registry
