URL: http://antibodyregistry.org/AB_626729
Proper Citation: Santa Cruz Biotechnology Cat# sc-7480, RRID:AB_626729
Target Antigen: BAX
Host Organism: mouse
Clonality: monoclonal
Comments: validation status unknown check with seller; recommendations: Flow Cytometry; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Immunoprecipitation; Western Blot; Western Blotting, Immunoprecipitation, Immunofluorescence, Immunohistochemistry(P), Flow Cytometry
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No rating or validation information has been found for Bax (B-9).
Issue
"..this reagent appears to be unreliable due to providing false-positive signals at the expected molecular weight of Bax in immunoblotting experiments and likewise provides false-positive signals in immunofluorescence-based detection of Bax expression. ... To our surprise, Bax Antibody (B-9) provided strong signals at an expected apparent molecular weight between 20 and 25 kDa in both wild-type and Bax/Bak deficient HCT 116 cells (Fig. 1A)..." Entrop et al 2024
Source: Antibody Registry
