URL: http://antibodyregistry.org/AB_572262
Proper Citation: (ImmunoStar Cat# 20079, RRID:AB_572262)
Target Antigen: Serotonin
Host Organism: goat
Clonality: polyclonal
Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, The 5-HT Goat Rabbit Antibody was raised against serotonin coupled to BSA with paraformaldehyde. The ImmunoStar serotonin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/400 - 1/800 in PBS/0.3% Triton X-100 - Cy3 Technique and 1/5000 - 1/10,000 in PBS/0.3% Triton X-100 - Bn/Av-HRP Technique. Staining is completely eliminated by pretreatment of the diluted antibody with 100 µg of serotonin/BSA conjugate.; Gene Symbol: Spl; Rated by ISCC, Intestinal Stem Cell Consortium (check ratings https://iscc.coh.org/)
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Source: Antibody Registry