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Mouse VEGF R2/Flk-1 Affinity Purified Polyclonal Ab antibody

RRID:AB_355500

Antibody ID

AB_355500

Target Antigen

Mouse VEGF R2/Flk-1 Affinity Purified Ab mouse

Proper Citation

(R and D Systems Cat# AF644, RRID:AB_355500)

Clonality

polyclonal antibody

Comments

vendor recommendations: IgG Block/Neutralize/Inhibit; Immunohistochemistry; Immunocytochemistry; Flow Cytometry; Western Blot; Flow Cytometry, Immunohistochemistry, Neutralization, Western Blot

Host Organism

goat

Vendor

R and D Systems

Cat Num

AF644

Publications that use this research resource

Hedgehog Pathway Drives Fusion-Negative Rhabdomyosarcoma Initiated From Non-myogenic Endothelial Progenitors.

  • Drummond CJ
  • Cancer Cell
  • 2018 Jan 8

Literature context:


Abstract:

Rhabdomyosarcoma (RMS) is a pediatric soft tissue sarcoma that histologically resembles embryonic skeletal muscle. RMS occurs throughout the body and an exclusively myogenic origin does not account for RMS occurring in sites devoid of skeletal muscle. We previously described an RMS model activating a conditional constitutively active Smoothened mutant (SmoM2) with aP2-Cre. Using genetic fate mapping, we show SmoM2 expression in Cre-expressing endothelial progenitors results in myogenic transdifferentiation and RMS. We show that endothelium and skeletal muscle within the head and neck arise from Kdr-expressing progenitors, and that hedgehog pathway activation results in aberrant expression of myogenic specification factors as a potential mechanism driving RMS genesis. These findings suggest that RMS can originate from aberrant development of non-myogenic cells.

Funding information:
  • NCI NIH HHS - K08 CA151649()
  • NCI NIH HHS - P30 CA021765()
  • NCI NIH HHS - R01 CA216344()
  • NIAID NIH HHS - R21AI094333(United States)

Microbiome Influences Prenatal and Adult Microglia in a Sex-Specific Manner.

  • Thion MS
  • Cell
  • 2018 Jan 25

Literature context:


Abstract:

Microglia are embryonically seeded macrophages that contribute to brain development, homeostasis, and pathologies. It is thus essential to decipher how microglial properties are temporally regulated by intrinsic and extrinsic factors, such as sexual identity and the microbiome. Here, we found that microglia undergo differentiation phases, discernable by transcriptomic signatures and chromatin accessibility landscapes, which can diverge in adult males and females. Remarkably, the absence of microbiome in germ-free mice had a time and sexually dimorphic impact both prenatally and postnatally: microglia were more profoundly perturbed in male embryos and female adults. Antibiotic treatment of adult mice triggered sexually biased microglial responses revealing both acute and long-term effects of microbiota depletion. Finally, human fetal microglia exhibited significant overlap with the murine transcriptomic signature. Our study shows that microglia respond to environmental challenges in a sex- and time-dependent manner from prenatal stages, with major implications for our understanding of microglial contributions to health and disease.

Funding information:
  • NIEHS NIH HHS - R01ES009949(United States)