URL: http://antibodyregistry.org/AB_2811180
Proper Citation: Mehlen, P., Cancer Res Centre of Lyon, France Cat# NP137, RRID:AB_2811180
Target Antigen: Netrin-1 (NET1)
Host Organism: mouse
Clonality: monoclonal
Comments: "HTP mice received eight injections (every 2 days; first injection in the presence of complete Freund adjuvant, the others with incomplete adjuvant) of 100 µg of netrin-1-Fc (Adipogen). Hybridoma fusion was performed 2 weeks after the first immunization (Abpro). Hybridoma supernatants were screened for specific monoclonal anti-netrin-1 antibodies using a dual-antigen ELISA assay (netrin-1-Fc and irrelevant Fc chimeric protein). A secondary ELISA-type assay was used to select monoclonal antibodies able to block the interaction of netrin-1 with DCC or UNC5H2. A murine monoclonal antibody (NET1-M-mAb) was then selected. Humanization of the antibody was performed by fusing in frame a combinatorial library of NET1-M-mAb antibodies to a validated collection of human frameworks (Bioatla Express Humanization). NET1-H-mAb (NP137) was provided by Netris Pharma."
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Source: Antibody Registry
