(Dr. Aimee Ryan; McGill University Health Centre; Quebec; Canada Cat# Ryan_bCA, RRID:AB_2728661)Copy Citation Copied
URL: http://antibodyregistry.org/AB_2728661
Proper Citation: (Dr. Aimee Ryan; McGill University Health Centre; Quebec; Canada Cat# Ryan_bCA, RRID:AB_2728661)
Target Antigen: PROP1
Host Organism: guinea pig
Clonality: polyclonal
Comments:
"PLoS One. 2009;4(3):e4815. doi: 10.1371/journal.pone.0004815. Epub 2009 Mar 13.
A GRFa2/Prop1/stem (GPS) cell niche in the pituitary.
Garcia-Lavandeira M1, Quereda V, Flores I, Saez C, Diaz-Rodriguez E, Japon MA, Ryan AK, Blasco MA, Dieguez C, Malumbres M, Alvarez CV.
Department of Physiology, School of Medicine, University of Santiago de Compostela, Santiago de Compostela, Spain."
"Guinea pig polyclonal antibody anti- Prop1 was made in house against the carboxy-terminal domain of mouse Prop-1. cDNA encoding amino acids 151 to 223 were cloned downstream of either a GST or a His-tag vector. Fusion proteins were expressed in BL21 E. coli and partially purified over glutathione agarose (Sigma) or Ni2+-NTA-agarose (Qiagen). Initial immunizations were performed with GST-Prop-1 fusion protein and the final boosts were performed with the His-Prop-1 fusion proteins. It has been already demonstrated that this antibody recognizes Prop1 transcription factor in mouse E12.5"
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Source: Antibody Registry
