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Donkey anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 488

RRID:AB_2556746

Antibody ID

AB_2556746

Target Antigen

Mouse IgG (H+L) Cross-Adsorbed mouse

Proper Citation

(Thermo Fisher Scientific Cat# SA5-10166, RRID:AB_2556746)

Clonality

polyclonal antibody

Reference

PMID:29071714

Comments

Applications: Flow (1:25 - 1:100), ICC (1:50-1:2,000), IF (1:50-1:2,000), IHC (1:50-1:2,000), IP (Assay Dependent), WB (1:5,000-1:20,000)

Host Organism

donkey

Vendor

Thermo Fisher Scientific Go To Vendor

Cat Num

SA5-10166

Publications that use this research resource

DNER and NFIA are expressed by developing and mature AII amacrine cells in the mouse retina.

  • Keeley PW
  • J. Comp. Neurol.
  • 2018 Feb 15

Literature context:


Abstract:

The present study has taken advantage of publicly available cell type specific mRNA expression databases in order to identify potential genes participating in the development of retinal AII amacrine cells. We profile two such genes, Delta/Notch-like EGF repeat containing (Dner) and nuclear factor I/A (Nfia), that are each heavily expressed in AII amacrine cells in the mature mouse retina, and which conjointly identify this retinal cell population in its entirety when using antibodies to DNER and NFIA. DNER is present on the plasma membrane, while NFIA is confined to the nucleus, consistent with known functions of each of these two proteins. DNER also identifies some other subsets of retinal ganglion and amacrine cell types, along with horizontal cells, while NFIA identifies a subset of bipolar cells as well as Muller glia and astrocytes. During early postnatal development, NFIA labels astrocytes on the day of birth, AII amacrine cells at postnatal (P) day 5, and Muller glia by P10, when horizontal cells also transiently exhibit NFIA immunofluorescence. DNER, by contrast, is present in ganglion and amacrine cells on P1, also labeling the horizontal cells by P10. Developing AII amacrine cells exhibit accumulating DNER labeling at the dendritic stalk, labeling that becomes progressively conspicuous by P10, as it is in maturity. This developmental time course is consistent with a prospective role for each gene in the differentiation of AII amacrine cells.