X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Rabbit Anti-Fis1 Polyclonal Antibody, Unconjugated

RRID:AB_2246809

Antibody ID

AB_2246809

Target Antigen

FIS1 human, mouse, rat

Proper Citation

(Santa Cruz Biotechnology Cat# sc-98900, RRID:AB_2246809)

Clonality

polyclonal antibody

Reference

PMID:29268097

Comments

Discontinued: 2016; validation status unknown check with seller; recommendations: western blot, ELISA, immunoprecipitation, immunocytochemistry

Host Organism

rabbit

Vendor

Santa Cruz Biotechnology

Cat Num

sc-98900

Publications that use this research resource

Drp1 Mitochondrial Fission in D1 Neurons Mediates Behavioral and Cellular Plasticity during Early Cocaine Abstinence.

  • Chandra R
  • Neuron
  • 2017 Dec 20

Literature context:


Abstract:

Altered brain energy homeostasis is a key adaptation occurring in the cocaine-addicted brain, but the effect of cocaine on the fundamental source of energy, mitochondria, is unknown. We demonstrate an increase of dynamin-related protein-1 (Drp1), the mitochondrial fission mediator, in nucleus accumbens (NAc) after repeated cocaine exposure and in cocaine-dependent individuals. Mdivi-1, a demonstrated fission inhibitor, blunts cocaine seeking and locomotor sensitization, while blocking c-Fos induction and excitatory input onto dopamine receptor-1 (D1) containing NAc medium spiny neurons (MSNs). Drp1 and fission promoting Drp1 are increased in D1-MSNs, consistent with increased smaller mitochondria in D1-MSN dendrites after repeated cocaine. Knockdown of Drp1 in D1-MSNs blocks drug seeking after cocaine self-administration, while enhancing the fission promoting Drp1 enhances seeking after long-term abstinence from cocaine. We demonstrate a role for altered mitochondrial fission in the NAc, during early cocaine abstinence, suggesting potential therapeutic treatment of disrupting mitochondrial fission in cocaine addiction.

Funding information:
  • NCI NIH HHS - R01 CA140198(United States)
  • NIAAA NIH HHS - R01 AA024845()
  • NIDA NIH HHS - R01 DA037257()
  • NIDA NIH HHS - R01 DA038613()
  • NIGMS NIH HHS - R25 GM055036()
  • NIGMS NIH HHS - SC2 GM109811()