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Parkin (Prk8) Mouse monoclonal Antibody

RRID:AB_2159920

Antibody ID

AB_2159920

Target Antigen

Parkin (Prk8) See NCBI gene human, mouse, rat

Proper Citation

(Cell Signaling Technology Cat# 4211, RRID:AB_2159920)

Clonality

monoclonal antibody

Comments

Applications: W, IP

Clone ID

Prk8

Host Organism

mouse

Vendor

Cell Signaling Technology

Cat Num

4211 also 4211S, 4211T

Publications that use this research resource

UBE2M Is a Stress-Inducible Dual E2 for Neddylation and Ubiquitylation that Promotes Targeted Degradation of UBE2F.

  • Zhou W
  • Mol. Cell
  • 2018 Jun 21

Literature context:


Abstract:

UBE2M and UBE2F are two family members of neddylation E2 conjugating enzyme that, together with E3s, activate CRLs (Cullin-RING Ligases) by catalyzing cullin neddylation. However, whether and how two E2s cross-talk with each other are largely unknown. Here, we report that UBE2M is a stress-inducible gene subjected to cis-transactivation by HIF-1 and AP1, and MLN4924, a small molecule inhibitor of E1 NEDD8-activating enzyme (NAE), upregulates UBE2M via blocking degradation of HIF-1α and c-JUN. UBE2M is a dual E2 for targeted ubiquitylation and degradation of UBE2F, acting as a neddylation E2 to activate CUL3-Keap1 E3 under physiological conditions but as a ubiquitylation E2 for Parkin-DJ-1 E3 under stressed conditions. UBE2M-induced UBE2F degradation leads to CRL5 inactivation and subsequent NOXA accumulation to suppress the growth of lung cancer cells. Collectively, our study establishes a negative regulatory axis between two neddylation E2s with UBE2M ubiquitylating UBE2F, and two CRLs with CRL3 inactivating CRL5.

Funding information:
  • NCI NIH HHS - R01 CA156744()
  • NCI NIH HHS - R01 CA171277()
  • NCI NIH HHS - R01-CA078461(United States)

Parkinson Sac Domain Mutation in Synaptojanin 1 Impairs Clathrin Uncoating at Synapses and Triggers Dystrophic Changes in Dopaminergic Axons.

  • Cao M
  • Neuron
  • 2017 Feb 22

Literature context:


Abstract:

Synaptojanin 1 (SJ1) is a major presynaptic phosphatase that couples synaptic vesicle endocytosis to the dephosphorylation of PI(4,5)P2, a reaction needed for the shedding of endocytic factors from their membranes. While the role of SJ1's 5-phosphatase module in this process is well recognized, the contribution of its Sac phosphatase domain, whose preferred substrate is PI4P, remains unclear. Recently a homozygous mutation in its Sac domain was identified in early-onset parkinsonism patients. We show that mice carrying this mutation developed neurological manifestations similar to those of human patients. Synapses of these mice displayed endocytic defects and a striking accumulation of clathrin-coated intermediates, strongly implicating Sac domain's activity in endocytic protein dynamics. Mutant brains had elevated auxilin (PARK19) and parkin (PARK2) levels. Moreover, dystrophic axonal terminal changes were selectively observed in dopaminergic axons in the dorsal striatum. These results strengthen evidence for a link between synaptic endocytic dysfunction and Parkinson's disease.

Funding information:
  • NCATS NIH HHS - UL1 TR001863()
  • NIDA NIH HHS - P30 DA018343()
  • NIGMS NIH HHS - P41 GM103412()
  • NINDS NIH HHS - R01 NS036251()
  • NINDS NIH HHS - R01 NS036942()
  • NINDS NIH HHS - R37 NS036251()
  • NINDS NIH HHS - R37 NS036942()

Prohibitin 2 Is an Inner Mitochondrial Membrane Mitophagy Receptor.

  • Wei Y
  • Cell
  • 2017 Jan 12

Literature context:


Abstract:

The removal of unwanted or damaged mitochondria by autophagy, a process called mitophagy, is essential for key events in development, cellular homeostasis, tumor suppression, and prevention of neurodegeneration and aging. However, the precise mechanisms of mitophagy remain uncertain. Here, we identify the inner mitochondrial membrane protein, prohibitin 2 (PHB2), as a crucial mitophagy receptor involved in targeting mitochondria for autophagic degradation. PHB2 binds the autophagosomal membrane-associated protein LC3 through an LC3-interaction region (LIR) domain upon mitochondrial depolarization and proteasome-dependent outer membrane rupture. PHB2 is required for Parkin-induced mitophagy in mammalian cells and for the clearance of paternal mitochondria after embryonic fertilization in C. elegans. Our findings pinpoint a conserved mechanism of eukaryotic mitophagy and demonstrate a function of prohibitin 2 that may underlie its roles in physiology, aging, and disease.

Funding information:
  • NCI NIH HHS - P30 CA142543()
  • NCI NIH HHS - R01 CA109618()
  • NIAID NIH HHS - K08 AI099150()