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Stat5 (pY694) antibody

RRID:AB_1645546

Antibody ID

AB_1645546

Target Antigen

Stat5 (pY694) human

Proper Citation

(BD Biosciences Cat# 560117, RRID:AB_1645546)

Clonality

monoclonal antibody

Comments

Intracellular staining (flow Cytotoxicityometry)

Host Organism

mouse

Vendor

BD Biosciences Go To Vendor

Cat Num

560117

Publications that use this research resource

Gli1+ Mesenchymal Stromal Cells Are a Key Driver of Bone Marrow Fibrosis and an Important Cellular Therapeutic Target.

  • Schneider RK
  • Cell Stem Cell
  • 2017 Jun 1

Literature context:


Abstract:

Bone marrow fibrosis (BMF) develops in various hematological and non-hematological conditions and is a central pathological feature of myelofibrosis. Effective cell-targeted therapeutics are needed, but the cellular origin of BMF remains elusive. Here, we show using genetic fate tracing in two murine models of BMF that Gli1+ mesenchymal stromal cells (MSCs) are recruited from the endosteal and perivascular niche to become fibrosis-driving myofibroblasts in the bone marrow. Genetic ablation of Gli1+ cells abolished BMF and rescued bone marrow failure. Pharmacological targeting of Gli proteins with GANT61 inhibited Gli1+ cell expansion and myofibroblast differentiation and attenuated fibrosis severity. The same pathway is also active in human BMF, and Gli1 expression in BMF significantly correlates with the severity of the disease. In addition, GANT61 treatment reduced the myofibroblastic phenotype of human MSCs isolated from patients with BMF, suggesting that targeting of Gli proteins could be a relevant therapeutic strategy.