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Mouse Anti-MEK-1 / 2 Monoclonal Antibody, Unconjugated, Clone 9G3

RRID:AB_1126111

Antibody ID

AB_1126111

Target Antigen

MAP2K1, MAP2K2 human, mouse, rat

Proper Citation

(Santa Cruz Biotechnology Cat# sc-81504, RRID:AB_1126111)

Clonality

monoclonal antibody

Reference

PMID:28414095

Comments

validation status unknown check with seller; recommendations: Western Blot; Western Blotting, Immunoprecipitation

Clone ID

9G3

Host Organism

mouse

Vendor

Santa Cruz Biotechnology

Cat Num

sc-81504

Publications that use this research resource

Crosstalk control and limits of physiological c-Jun N-terminal kinase activity for cell viability and neurite stability in differentiated PC12 cells.

  • Waetzig V
  • Mol. Cell. Neurosci.
  • 2018 Apr 24

Literature context:


Abstract:

The c-Jun N-terminal kinases (JNKs) are important mediators of cell viability and structural integrity in postmitotic neurons, which is required for maintaining synaptic connections and neural plasticity. In the present study, we chose differentiated PC12 cells as a well-characterised neuronal model system to selectively examine the regulation of basal JNK activity by extracellular signal-regulated kinase 1/2 (ERK1/2) and Akt. We detected a complex interaction between the kinases to prevent cell death and neurite loss. Especially the appropriate level of JNK activation determined cellular survival. Basal activity of ERK1/2 attenuated the potentiation of JNK phosphorylation and thereby the induction of apoptosis. Importantly, when JNK activity was too low, cell viability and the number of neurite-bearing cells also decreased, even though the activation of ERK1/2 was enhanced. In this case, the JNK-mediated survival signals via activating transcription factor-3 (ATF3) were inhibited. Furthermore, the phosphorylation of ERK1/2 induced by the JNK inhibitor SP600125 inhibited the basal activity of Akt, which normally supported cell viability. Thus, controlling JNK activity is crucial to promote survival and neurite stability of differentiated neuronal cells.