URL: http://antibodyregistry.org/AB_91789
Proper Citation: (Millipore Cat# AB5320, RRID:AB_91789)
Target Antigen: NG2 Chondroitin Sulfate Proteoglycan
Host Organism: rabbit
Clonality: polyclonal
Comments:
Applications: IHC, WB
Consolidation on 2/2024: AB_11213678
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No rating or validation information has been found for Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody.
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The authors state: "Depending on secondary antibody, there was false positive Rab6A staining selectively in microglial cells (1–3 larger granula in many cells) present also in the controls (see Figure S8). Only with biotinylated horse anti-goat (followed by CY3-conjugated streptavidin) and donkey anti-mouse Alexa 647 was this non-specific, selective staining prevented. Virtually all non-astrocytic cells examined are negative for Rab6A: 99% of NG2 cells; 100% of microglia; and 98.3% of oligodendrocytes from cortex, 99.5% from corpus callosum, and 100% from hippocampus (Figure 5), and no Rab6A+ neurons were found (Figure S9)."
Source: Antibody Registry
