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CD127 antibody

RRID:AB_11151911

Antibody ID

AB_11151911

Target Antigen

CD127 human

Proper Citation

(BD Biosciences Cat# 562436, RRID:AB_11151911)

Clonality

monoclonal antibody

Reference

PMID:29290585

Comments

Flow cytometry

Host Organism

mouse

Vendor

BD Biosciences Go To Vendor

Cat Num

562436

Publications that use this research resource

A Human IPS Model Implicates Embryonic B-Myeloid Fate Restriction as Developmental Susceptibility to B Acute Lymphoblastic Leukemia-Associated ETV6-RUNX1.

  • Böiers C
  • Dev. Cell
  • 2018 Feb 5

Literature context:


Abstract:

ETV6-RUNX1 is associated with childhood acute B-lymphoblastic leukemia (cALL) functioning as a first-hit mutation that initiates a clinically silent pre-leukemia in utero. Because lineage commitment hierarchies differ between embryo and adult, and the impact of oncogenes is cell-context dependent, we hypothesized that the childhood affiliation of ETV6-RUNX1 cALL reflects its origins in a progenitor unique to embryonic life. We characterize the first emerging B cells in first-trimester human embryos, identifying a developmentally restricted CD19-IL-7R+ progenitor compartment, which transitions from a myeloid to lymphoid program during ontogeny. This developmental series is recapitulated in differentiating human pluripotent stem cells (hPSCs), thereby providing a model for the initiation of cALL. Genome-engineered hPSCs expressing ETV6-RUNX1 from the endogenous ETV6 locus show expansion of the CD19-IL-7R+ compartment, show a partial block in B lineage commitment, and produce proB cells with aberrant myeloid gene expression signatures and potential: features (collectively) consistent with a pre-leukemic state.