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Mitofilin antibody [2E4AD5]

RRID:AB_10859613

Antibody ID

AB_10859613

Target Antigen

Mitofilin antibody [2E4AD5] human, mouse, rat, bovine, human, mouse, rat, cow

Proper Citation

(Abcam Cat# ab110329, RRID:AB_10859613)

Clonality

monoclonal antibody

Reference

PMID:29456190

Comments

validation status unknown, seller recommendations provided in 2012: Flow Cyt, ICC/IF, IHC-P, In-Cell ELISA, IP, WB; Immunohistochemistry; Immunofluorescence; Immunoprecipitation; ELISA; Immunocytochemistry; Immunohistochemistry - fixed; Flow Cytometry; Western Blot

Host Organism

mouse

Vendor

Abcam

Cat Num

ab110329

Publications that use this research resource

PINK1 Phosphorylates MIC60/Mitofilin to Control Structural Plasticity of Mitochondrial Crista Junctions.

  • Tsai PI
  • Mol. Cell
  • 2018 Mar 1

Literature context:


Abstract:

Mitochondrial crista structure partitions vital cellular reactions and is precisely regulated by diverse cellular signals. Here, we show that, in Drosophila, mitochondrial cristae undergo dynamic remodeling among distinct subcellular regions and the Parkinson's disease (PD)-linked Ser/Thr kinase PINK1 participates in their regulation. Mitochondria increase crista junctions and numbers in selective subcellular areas, and this remodeling requires PINK1 to phosphorylate the inner mitochondrial membrane protein MIC60/mitofilin, which stabilizes MIC60 oligomerization. Expression of MIC60 restores crista structure and ATP levels of PINK1-null flies and remarkably rescues their behavioral defects and dopaminergic neurodegeneration. In an extension to human relevance, we discover that the PINK1-MIC60 pathway is conserved in human neurons, and expression of several MIC60 coding variants in the mitochondrial targeting sequence found in PD patients in Drosophila impairs crista junction formation and causes locomotion deficits. These findings highlight the importance of maintenance and plasticity of crista junctions to cellular homeostasis in vivo.

Funding information:
  • NIGMS NIH HHS - T32 GM007337(United States)