URL: http://antibodyregistry.org/AB_10603025
Proper Citation: (Sigma-Aldrich Cat# HPA028835, RRID:AB_10603025)
Target Antigen: SLC10A4 antibody produced in rabbit
Host Organism: rabbit
Clonality: polyclonal
Comments: Vendor recommendations: immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, protein array: suitable, immunoblotting: suitable; Immunohistochemistry; Western Blot; Other
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- Antibody validation available from The Human Protein Atlas - Human Protein Atlas https://www.proteinatlas.org/search/HPA028835
Possibly Non-Specific
Extracted Antibody Information: "The non-specific binding sites were blocked with 1% bovine serum albumin (BSA) plus 4% goat serum (Sigma-Aldrich) in PBS for 30 min at room temperature. Then, the cells were incubated with the primary antibodies, rabbit anti-Slc10a4 (antibody 1338 C, 1:1,000 dilution, see [13, 14] ), Sigma Prestige rabbit anti-SLC10A4 (1:500 dilution) [Sigma-Aldrich Cat# HPA028835 RRID:AB_10603025],"
Extracted Specificity Statement: "They used the human medulloblastoma cell line TE671 in which they localized the SLC10A4 protein by immunofluorescence and Western Blot analysis with a commercial anti-SLC10A4 antibody and showed that pre-incubation of the cells with 1U/200 µl of thrombin for 3 h increased the cellular uptake of taurocholic acid and lithocholic acid [18]. However, appropriate controls demonstrating specificity of the antibody were not provided and the authors failed to seriously show saturable transport kinetics for this uptake. Furthermore, bioinformatics analysis (Expasy Peptide Cutter, http://www.web.expasy.org/peptide_cutter/) indicates that the mouse and rat SLC10A4 proteins can be cleaved by thrombin at amino acid position 87, but not the human SLC10A4 protein."
Data was mined by Antibody Watch (https://arxiv.org/pdf/2008.01937.pdf), from PMID:26084360
Source: Antibody Registry
