URL: http://www.ncbi.nlm.nih.gov/tools/primer-blast/
Proper Citation: Primer-BLAST (RRID:SCR_003095)
Description: A tool to design target-specific primers for polymerase chain reaction (PCR). It uses Primer3 to design PCR primers and then uses BLAST and global alignment algorithm to screen primers against user-selected database in order to avoid primer pairs (all combinations including forward-reverse primer pair, forward-forward as well as reverse-reverse pairs) that can cause non-specific amplifications.
Abbreviations: Primer-BLAST
Resource Type: analysis service resource, data analysis service, production service resource, service resource
Defining Citation: PMID:22708584
Keywords: primer, blast, pcr target, polymerase chain reaction, primer design
Availability: Free, Freely available
Resource Name: Primer-BLAST
Resource ID: SCR_003095
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Source: SciCrunch Registry (RRID:SCR_005400)
Description: Interactive portal for finding and submitting biomedical resources. Resources within SciCrunch have assigned RRIDs which are used to cite resources in scientific manuscripts. SciCrunch Registry, formerly NIF Registry, provides resources catalog. Allows to add new resources. Allows edit existing resources after registration. Curators are tasked with identifying and registering resources, examining data, writing configuration files to index and display data and keeping contents current.
URL: https://rrid.site/rin/sources/SCR_005400
