High-resolution mapping of cell cycle dynamics during steady-state T cell development and regeneration in vivo.
Control of cell proliferation is critical for the lymphocyte life cycle. However, little is known about how stage-specific alterations in cell cycle behavior drive proliferation dynamics during T cell development. Here, we employed in vivo dual-nucleoside pulse labeling combined with the determination of DNA replication over time as well as fluorescent ubiquitination-based cell cycle indicator mice to establish a quantitative high-resolution map of cell cycle kinetics of thymocytes. We developed an agent-based mathematical model of T cell developmental dynamics. To generate the capacity for proliferative bursts, cell cycle acceleration followed a "stretch model" characterized by the simultaneous and proportional contraction of both G1 and S phases. Analysis of cell cycle phase dynamics during regeneration showed tailored adjustments of cell cycle phase dynamics. Taken together, our results highlight intrathymic cell cycle regulation as an adjustable system to maintain physiologic tissue homeostasis and foster our understanding of dysregulation of the T cell developmental program.
Pubmed ID: 39756036 RIS Download
Research resources used in this publication
Additional research tools detected in this publication
None foundAntibodies used in this publication
- PerCP/Cyanine5.5 anti-mouse/human CD44 (RRID:AB_2076204)
- PerCP/Cyanine5.5 anti-mouse CD25 (RRID:AB_893288)
- APC/Cyanine7 anti-mouse/human CD44 (RRID:AB_830785)
- Brilliant Violet 421(TM) anti-mouse CD25 (RRID:AB_11203373)
- PE anti-mouse CD28 (RRID:AB_604078)
- PE/Cyanine7 anti-mouse CD8a (RRID:AB_312761)
- PE anti-mouse CD69 (RRID:AB_313111)
- FITC anti-BrdU (RRID:AB_2564481)
- PerCP/Cyanine5.5 anti-mouse CD4 (RRID:AB_893324)
- PE/Cyanine7 anti-mouse CD4 (RRID:AB_312707)
- Brilliant Violet 510(TM) anti-mouse CD8a (RRID:AB_2561389)
- APC/Cyanine7 anti-mouse CD117 (c-kit) (RRID:AB_1626278)
- APC/Cyanine7 anti-mouse TCR beta chain (RRID:AB_893624)
- APC anti-mouse CD71 (RRID:AB_2728134)
- Brilliant Violet 421(TM) anti-mouse TCR beta chain (RRID:AB_2562562)
- APC anti-mouse CD69 (RRID:AB_492843)
- PE/Cyanine7 anti-mouse TCR beta chain (RRID:AB_893625)
Associated grants
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This is a list of tools and resources that we have found mentioned in this publication.
PerCP/Cyanine5.5 anti-mouse/human CD44 (antibody)
RRID:AB_2076204
This monoclonal targets CD44
View all literature mentionsPerCP/Cyanine5.5 anti-mouse CD25 (antibody)
RRID:AB_893288
This monoclonal targets CD25
View all literature mentionsAPC/Cyanine7 anti-mouse/human CD44 (antibody)
RRID:AB_830785
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View all literature mentionsBrilliant Violet 421(TM) anti-mouse CD25 (antibody)
RRID:AB_11203373
This monoclonal targets CD25
View all literature mentionsPE anti-mouse CD28 (antibody)
RRID:AB_604078
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View all literature mentionsPE/Cyanine7 anti-mouse CD8a (antibody)
RRID:AB_312761
This monoclonal targets CD8a
View all literature mentionsPE anti-mouse CD69 (antibody)
RRID:AB_313111
This monoclonal targets CD69
View all literature mentionsPerCP/Cyanine5.5 anti-mouse CD4 (antibody)
RRID:AB_893324
This monoclonal targets CD4
View all literature mentionsPE/Cyanine7 anti-mouse CD4 (antibody)
RRID:AB_312707
This monoclonal targets CD4
View all literature mentionsBrilliant Violet 510(TM) anti-mouse CD8a (antibody)
RRID:AB_2561389
This monoclonal targets CD8a
View all literature mentionsAPC/Cyanine7 anti-mouse CD117 (c-kit) (antibody)
RRID:AB_1626278
This monoclonal targets CD117
View all literature mentionsAPC/Cyanine7 anti-mouse TCR beta chain (antibody)
RRID:AB_893624
This monoclonal targets TCR beta chain
View all literature mentionsAPC anti-mouse CD71 (antibody)
RRID:AB_2728134
This monoclonal targets CD71
View all literature mentionsBrilliant Violet 421(TM) anti-mouse TCR beta chain (antibody)
RRID:AB_2562562
This monoclonal targets TCR beta chain
View all literature mentionsAPC anti-mouse CD69 (antibody)
RRID:AB_492843
This monoclonal targets CD69
View all literature mentionsPE/Cyanine7 anti-mouse TCR beta chain (antibody)
RRID:AB_893625
This monoclonal targets TCR beta chain
View all literature mentionsFlowJo (software resource)
RRID:SCR_008520
Software for single-cell flow cytometry analysis. Its functions include management, display, manipulation, analysis and publication of the data stream produced by flow and mass cytometers.
View all literature mentionsGraphPad Prism (software resource)
RRID:SCR_002798
Statistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
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