Generation of three human induced pluripotent stem cell lines from retinitis pigmentosa 25 patient and two carriers but asymptomatic daughters.
Retinitis Pigmentosa type 25 (RP25) is a form of inherited retinal dystrophy characterized by a progressive loss of rod photoreceptors, subsequent degeneration of cone photoreceptors, and eventually, the retinal pigment epithelium. Caused by mutations in the EYS gene, it is believed to be critical for the structural and functional integrity of the retina. Using a non-integrative RNA reprogramming method, we have generated human induced pluripotent stem cell (hiPSC) lines from RP25 patient and from carriers but asymptomatic daughters. These three hiPSC lines maintain a normal karyotype, exhibit pluripotency gene expression, and can differentiate into the three germ layers.
Pubmed ID: 39742628 RIS Download
Research resources used in this publication
None foundAdditional research tools detected in this publication
None foundAntibodies used in this publication
- Cy™3 AffiniPure Donkey Anti-Goat IgG (H+L) (RRID:AB_2307351)
- Cy3-AffiniPure Donkey Anti-Mouse IgM, µ Chain Specific (min X Hu,Bov,Hrs,Rat Sr Prot) (RRID:AB_2340812)
- Fluorescein (FITC)-AffiniPure Donkey Anti-Rat IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rb,Shp Sr Prot) (RRID:AB_2340652)
- Alpha-Sarcomeric Actin (RRID:AB_476695)
- Sox 17, SRY (sex determining region Y)-box 17 (RRID:AB_2195648)
- Rabbit Anti-Pax-6 Polyclonal Antibody, Unconjugated (RRID:AB_291612)
- Alpha-1-Fetoprotein Pathology Antibody (RRID:AB_2650473)
- Alexa Fluor 488-AffiniPure Fab Fragment Donkey Anti-Mouse IgG (H+L) (RRID:AB_2340851)
- Human Nanog Antibody (RRID:AB_355097)
- Mouse Anti-Human SSEA-4 (carbohydrate from human embryonal carcinoma) Monoclonal Antibody, Unconjugated (RRID:AB_528477)
- Neuronal Class III beta-Tubulin (TUJ1) Monoclonal Antibody, Purified (RRID:AB_2313773)
- Anti-TRA-1-81, clone TRA-1-81 (RRID:AB_177638)
- SSEA-3 (carbohydrate from 4-8 cell mouse embryos) antibody - Solter, D. / Knowles, B.B; (RRID:AB_528476)
- Oct3/4 Antibody (C-10) (RRID:AB_628051)
Associated grants
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This is a list of tools and resources that we have found mentioned in this publication.
Cy™3 AffiniPure Donkey Anti-Goat IgG (H+L) (antibody)
RRID:AB_2307351
This polyclonal targets IgG (H+L)
View all literature mentionsCy3-AffiniPure Donkey Anti-Mouse IgM, µ Chain Specific (min X Hu,Bov,Hrs,Rat Sr Prot) (antibody)
RRID:AB_2340812
This unknown targets Mouse IgM,
View all literature mentionsFluorescein (FITC)-AffiniPure Donkey Anti-Rat IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rb,Shp Sr Prot) (antibody)
RRID:AB_2340652
This unknown targets Rat IgG (H+L)
View all literature mentionsAlpha-Sarcomeric Actin (antibody)
RRID:AB_476695
This monoclonal targets Purified rabbit striated muscle actin used as immunogen
View all literature mentionsSox 17, SRY (sex determining region Y)-box 17 (antibody)
RRID:AB_2195648
This unknown targets ND
View all literature mentionsRabbit Anti-Pax-6 Polyclonal Antibody, Unconjugated (antibody)
RRID:AB_291612
This polyclonal targets Pax-6
View all literature mentionsAlpha-1-Fetoprotein Pathology Antibody (antibody)
RRID:AB_2650473
This polyclonal targets Alpha-1-fetoprotein
View all literature mentionsAlexa Fluor 488-AffiniPure Fab Fragment Donkey Anti-Mouse IgG (H+L) (antibody)
RRID:AB_2340851
This unknown targets Mouse IgG (H+L)
View all literature mentionsHuman Nanog Antibody (antibody)
RRID:AB_355097
This polyclonal targets Nanog
View all literature mentionsMouse Anti-Human SSEA-4 (carbohydrate from human embryonal carcinoma) Monoclonal Antibody, Unconjugated (antibody)
RRID:AB_528477
This monoclonal targets Mouse Human SSEA-4 (carbohydrate from human embryonal carcinoma)
View all literature mentionsNeuronal Class III beta-Tubulin (TUJ1) Monoclonal Antibody, Purified (antibody)
RRID:AB_2313773
This monoclonal targets Neuronal Class III beta-Tubulin (TUJ1) Purified
View all literature mentionsAnti-TRA-1-81, clone TRA-1-81 (antibody)
RRID:AB_177638
This monoclonal targets TRA-1-81 clone TRA-1-81
View all literature mentionsSSEA-3 (carbohydrate from 4-8 cell mouse embryos) antibody - Solter, D. / Knowles, B.B; (antibody)
RRID:AB_528476
This monoclonal targets SSEA-3 (carbohydrate from 4-8 cell mouse embryos)
View all literature mentionsOct3/4 Antibody (C-10) (antibody)
RRID:AB_628051
This monoclonal targets Oct-3/4
View all literature mentions
