Tyzzerella nexilis strains enriched in mobile genetic elements are involved in progressive multiple sclerosis.
Multiple sclerosis (MS) is an autoimmune-demyelinating disease with an inflammatory pathology formed by self-reactive lymphocytes with activated glial cells. Progressive MS, characterized by resistance to medications, significantly differs from the non-progressive form in gut microbiome profiles. After confirming an increased abundance of "Tyzzerella nexilis" in various cohorts of progressive MS, we identified a distinct cluster of T. nexilis strains enriched in progressive MS based on long-read metagenomics. The distinct T. nexilis cluster is characterized by a large number of mobile genetic elements (MGEs) and a lack of defense systems against MGEs. Microbial genes for sulfate reduction and flagella formation with pathogenic implications are specific to this cluster. Moreover, these flagellar genes are encoded on MGEs. Mono-colonization with MGE-enriched T. nexilis made germ-free mice more susceptible to experimental autoimmune encephalomyelitis. These results indicate that the progression of MS may be promoted by MGE-enriched T. nexilis with potentially pathogenic properties.
Pubmed ID: 39341204 RIS Download
Research resources used in this publication
None foundAdditional research tools detected in this publication
None foundAntibodies used in this publication
- Purified anti-mouse CD28 (RRID:AB_312866)
- PE anti-mouse/human CD11b (RRID:AB_312791)
- APC anti-mouse CD11c (RRID:AB_313778)
- Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) Clone 2.4G2 (RRID:AB_394656)
- CD4 Monoclonal Antibody (RM4-5), APC-eFluor™ 780, eBioscience (RRID:AB_1272183)
- FOXP3 Monoclonal Antibody (FJK-16s), PE, eBioscience (RRID:AB_465936)
- FITC anti-mouse IFN-gamma (RRID:AB_315400)
- APC anti-mouse CD25 (RRID:AB_2280288)
- PE/Cyanine7 anti-mouse IL-17A (RRID:AB_2125010)
- BV421 Mouse Anti-Mouse RORγt Antibody (RRID:AB_2687545)
- PerCP/Cyanine5.5 anti-mouse CD8a (RRID:AB_2075238)
- Alexa Fluor(R) 647 anti-mouse CD3 (RRID:AB_389323)
- PE/Cyanine7 anti-mouse TCR beta chain (RRID:AB_893625)
- FITC anti-mouse/human CD44 (RRID:AB_493685)
- CD4 (RRID:AB_1937323)
- Human CXCR5 Allophycocyanin MAb (Clone 51505) (RRID:AB_357109)
- Mouse Anti-Human Cxcr3 Monoclonal antibody, Fluorescein Conjugated, Clone 49801 (RRID:AB_357060)
- PerCP/Cyanine5.5 anti-mouse CD4 (RRID:AB_893330)
- Mouse Anti-Human CD45RA Monoclonal Antibody, APC-H7 Conjugated, Clone HI100 (RRID:AB_1727497)
- PE anti-mouse CD62L (RRID:AB_313095)
- Brilliant Violet 421(TM) anti-human CD127 (IL-7Ralpha) (RRID:AB_10960140)
- PE/Cyanine7 anti-human CD25 (RRID:AB_314282)
- PerCP/Cyanine5.5 anti-human CD196 (CCR6) (RRID:AB_10918985)
- PE anti-human CD3 (RRID:AB_2564150)
Associated grants
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This is a list of tools and resources that we have found mentioned in this publication.
Purified anti-mouse CD28 (antibody)
RRID:AB_312866
This monoclonal targets CD28
View all literature mentionsPE anti-mouse/human CD11b (antibody)
RRID:AB_312791
This monoclonal targets CD11b
View all literature mentionsAPC anti-mouse CD11c (antibody)
RRID:AB_313778
This monoclonal targets CD11c
View all literature mentionsPurified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) Clone 2.4G2 (antibody)
RRID:AB_394656
This monoclonal targets CD16/CD32
View all literature mentionsCD4 Monoclonal Antibody (RM4-5), APC-eFluor™ 780, eBioscience (antibody)
RRID:AB_1272183
This monoclonal targets CD4
View all literature mentionsFOXP3 Monoclonal Antibody (FJK-16s), PE, eBioscience (antibody)
RRID:AB_465936
This monoclonal targets FOXP3
View all literature mentionsFITC anti-mouse IFN-gamma (antibody)
RRID:AB_315400
This monoclonal targets IFN-gamma
View all literature mentionsAPC anti-mouse CD25 (antibody)
RRID:AB_2280288
This monoclonal targets CD25
View all literature mentionsPE/Cyanine7 anti-mouse IL-17A (antibody)
RRID:AB_2125010
This monoclonal targets IL-17A
View all literature mentionsBV421 Mouse Anti-Mouse RORγt Antibody (antibody)
RRID:AB_2687545
This monoclonal targets Mouse RORγt
View all literature mentionsPerCP/Cyanine5.5 anti-mouse CD8a (antibody)
RRID:AB_2075238
This monoclonal targets CD8a
View all literature mentionsAlexa Fluor(R) 647 anti-mouse CD3 (antibody)
RRID:AB_389323
This monoclonal targets CD3
View all literature mentionsPE/Cyanine7 anti-mouse TCR beta chain (antibody)
RRID:AB_893625
This monoclonal targets TCR beta chain
View all literature mentionsFITC anti-mouse/human CD44 (antibody)
RRID:AB_493685
This monoclonal targets CD44
View all literature mentionsHuman CXCR5 Allophycocyanin MAb (Clone 51505) (antibody)
RRID:AB_357109
This monoclonal targets Human CXCR5 Allophycocyanin MAb (Clone 51505)
View all literature mentionsMouse Anti-Human Cxcr3 Monoclonal antibody, Fluorescein Conjugated, Clone 49801 (antibody)
RRID:AB_357060
This monoclonal targets Human CXCR3
View all literature mentionsPerCP/Cyanine5.5 anti-mouse CD4 (antibody)
RRID:AB_893330
This monoclonal targets CD4
View all literature mentionsMouse Anti-Human CD45RA Monoclonal Antibody, APC-H7 Conjugated, Clone HI100 (antibody)
RRID:AB_1727497
This monoclonal targets Human CD45RA
View all literature mentionsPE anti-mouse CD62L (antibody)
RRID:AB_313095
This monoclonal targets CD62L
View all literature mentionsBrilliant Violet 421(TM) anti-human CD127 (IL-7Ralpha) (antibody)
RRID:AB_10960140
This monoclonal targets CD127
View all literature mentionsPE/Cyanine7 anti-human CD25 (antibody)
RRID:AB_314282
This monoclonal targets CD25
View all literature mentionsPerCP/Cyanine5.5 anti-human CD196 (CCR6) (antibody)
RRID:AB_10918985
This monoclonal targets CD196
View all literature mentionsPE anti-human CD3 (antibody)
RRID:AB_2564150
This monoclonal targets CD3
View all literature mentions
