Hox gene activity directs physical forces to differentially shape chick small and large intestinal epithelia.
Hox transcription factors play crucial roles in organizing developmental patterning across metazoa, but how these factors trigger regional morphogenesis has largely remained a mystery. In the developing gut, Hox genes help demarcate identities of intestinal subregions early in embryogenesis, which ultimately leads to their specialization in both form and function. Although the midgut forms villi, the hindgut develops sulci that resolve into heterogeneous outgrowths. Combining mechanical measurements of the embryonic chick intestine and mathematical modeling, we demonstrate that the posterior Hox gene HOXD13 regulates biophysical phenomena that shape the hindgut lumen. We further show that HOXD13 acts through the transforming growth factor β (TGF-β) pathway to thicken, stiffen, and promote isotropic growth of the subepithelial mesenchyme-together, these features lead to hindgut-specific surface buckling. TGF-β, in turn, promotes collagen deposition to affect mesenchymal geometry and growth. We thus identify a cascade of events downstream of positional identity that direct posterior intestinal morphogenesis.
Pubmed ID: 39116876 RIS Download
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- NOT AN ANTIBODY Peroxidase-Streptavidin (RRID:AB_2337238)
- Biotin-SP-AffiniPure Goat Anti-Rabbit IgG (H+L) (RRID:AB_2337959)
- Alexa Fluor 488-AffiniPure Donkey Anti-Rabbit IgG (H+L) (RRID:AB_2313584)
- Alexa Fluor 647-AffiniPure Donkey Anti-Rabbit IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rat,Shp Sr Prot) (RRID:AB_2492288)
- Cy3-AffiniPure Donkey Anti-Mouse IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Rb,Shp Sr Prot) (RRID:AB_2340813)
- Rabbit Anti-Collagen I Polyclonal Antibody, Unconjugated (RRID:AB_731684)
- Phospho-Smad2 (Ser465/467) Antibody (RRID:AB_331673)
- Calponin 1 (D8L2T) XP® Rabbit mAb (RRID:AB_2798789)
- Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [1A4] (RRID:AB_2924381)
- Mouse Anti-Actin, alpha, smooth muscle Monoclonal Antibody, Fluorescein Conjugated, Clone 1A4 (RRID:AB_306359)
- Mouse Anti-Avian Myoblastosis Virus p19^(gag) Monoclonal Antibody, Unconjugated (RRID:AB_528098)
Associated grants
- Agency: NICHD NIH HHS, United States
Id: R01 HD089934
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NOT AN ANTIBODY Peroxidase-Streptavidin (antibody)
RRID:AB_2337238
This unknown targets Biotin
View all literature mentionsBiotin-SP-AffiniPure Goat Anti-Rabbit IgG (H+L) (antibody)
RRID:AB_2337959
This unknown targets Rabbit IgG (H+L)
View all literature mentionsAlexa Fluor 488-AffiniPure Donkey Anti-Rabbit IgG (H+L) (antibody)
RRID:AB_2313584
This polyclonal secondary targets IgG (H+L)
View all literature mentionsAlexa Fluor 647-AffiniPure Donkey Anti-Rabbit IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rat,Shp Sr Prot) (antibody)
RRID:AB_2492288
This polyclonal targets Rabbit IgG
View all literature mentionsCy3-AffiniPure Donkey Anti-Mouse IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Rb,Shp Sr Prot) (antibody)
RRID:AB_2340813
This unknown targets Mouse IgG (H+L)
View all literature mentionsRabbit Anti-Collagen I Polyclonal Antibody, Unconjugated (antibody)
RRID:AB_731684
This polyclonal targets Collagen I
View all literature mentionsPhospho-Smad2 (Ser465/467) Antibody (antibody)
RRID:AB_331673
This polyclonal targets Phospho-Smad2 (Ser465/467)
View all literature mentionsCalponin 1 (D8L2T) XP® Rabbit mAb (antibody)
RRID:AB_2798789
This monoclonal targets calponin 1
View all literature mentionsAlexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [1A4] (antibody)
RRID:AB_2924381
This monoclonal targets alpha smooth muscle Actin
View all literature mentionsMouse Anti-Actin, alpha, smooth muscle Monoclonal Antibody, Fluorescein Conjugated, Clone 1A4 (antibody)
RRID:AB_306359
This monoclonal targets alpha smooth muscle Actin
View all literature mentionsMouse Anti-Avian Myoblastosis Virus p19^(gag) Monoclonal Antibody, Unconjugated (antibody)
RRID:AB_528098
This monoclonal targets Mouse Avian Myoblastosis Virus p19^(gag)
View all literature mentions
