Monoacylglycerol acyltransferase-2 inhibits colorectal carcinogenesis in APCmin+/- mice.
Monoacylglycerol acyltransferase-2 (MOGAT2), encodes MOGAT enzyme in the re-synthesis of triacylglycerol and protects from metabolism disorders. While, its precise involvement in colorectal cancer (CRC) progression remains inadequately understood. Our study demonstrated that knockout of Mogat2 in Apcmin/+ mice expedited intestinal tumor growth and progression, indicating that Mogat2 plays a tumor-suppressing role in CRC. Mechanically, Mogat2 deletion resulted in a significant alter the gut microbiota, while Fecal Microbiota Transplantation (FMT) experiments demonstrated that the gut microbiota in Mogat2 deleted mice promoted tumor growth. Furthermore, we identified Mogat2 as a functional regulator suppressing CRC cell proliferation and tumor growth by inhibiting the NF-κB signaling pathway in vivo. Collectively, these results provide novel insights into the protective double roles of Mogat2, inhibiting of NF-κB pathway and keeping gut microbiota homeostasis in colorectal cancer, which may help the development of novel cancer treatments for CRC.
Pubmed ID: 39055928 RIS Download
Research resources used in this publication
Additional research tools detected in this publication
Antibodies used in this publication
- Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (RRID:AB_331284)
- Phospho-I B (Ser32) (14D4) Rabbit mAb (RRID:AB_561111)
- IκBα (44D4) Rabbit mAb (RRID:AB_10694416)
- NF- B1 p105/p50 Antibody (RRID:AB_330564)
- Ki-67 (D3B5) Rabbit mAb (Mouse Preferred; IHC Formulated) (RRID:AB_2620142)
- NF-kappaB p65 (D14E12) XP Rabbit mAb (RRID:AB_10859369)
- Alpha Tubulin antibody (RRID:AB_2210206)
- Anti-MGAT2 antibody (RRID:AB_3105860)
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