CT135 mediates the resistance of Chlamydia trachomatis to primate interferon gamma stimulated immune defenses.
Evading host innate immune defenses is a critical feature of Chlamydia trachomatis infections, and the mechanisms used by C. trachomatis to subvert these pathways are incompletely understood. We screened a library of chimeric C. trachomatis mutants for genetic factors important for interference with cell-autonomous immune defenses. Mutant strains with predicted truncations of the inclusion membrane protein CT135 were susceptible to interferon gamma-activated immunity in human cells. CT135 functions to prevent host-driven recruitment of ubiquitin and p62/SQSTM to the inclusion membrane. In a nonhuman primate model of C. trachomatis infection, a CT135-deficient strain was rapidly cleared, highlighting the importance of this virulence factor for C. trachomatis pathogenesis. Analysis of CT135 phenotypes in primary macaque cells revealed that cell-autonomous immune defenses against C. trachomatis are conserved between humans and nonhuman primates and connects mechanistic findings with in vivo infection outcomes.
Pubmed ID: 38947519 RIS Download
Research resources used in this publication
Additional research tools detected in this publication
None foundAntibodies used in this publication
- Recombinant Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (RRID:AB_2810880)
- Rabbit anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 594 (RRID:AB_2534109)
- Donkey Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor ?? 594 Conjugated (RRID:AB_141637)
- Donkey Anti-Goat IgG (H+L) Antibody, Alexa Fluor 488 Conjugated (RRID:AB_2534102)
- Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (RRID:AB_2534069)
- CALCOCO2 antibody (RRID:AB_1640255)
- Anti-Mono- and polyubiquitinylated conjugates Monoclonal Antibody, Unconjugated, Clone FK2 (RRID:AB_2051891)
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This is a list of tools and resources that we have found mentioned in this publication.
Recombinant Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (antibody)
RRID:AB_2810880
This recombinant targets SQSTM1 / p62
View all literature mentionsRabbit anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 594 (antibody)
RRID:AB_2534109
This polyclonal secondary targets IgG (H+L)
View all literature mentionsDonkey Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor ?? 594 Conjugated (antibody)
RRID:AB_141637
This unknown targets Rabbit IgG (H+L)
View all literature mentionsDonkey Anti-Goat IgG (H+L) Antibody, Alexa Fluor 488 Conjugated (antibody)
RRID:AB_2534102
This unknown targets IgG (H+L)
View all literature mentionsGoat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (antibody)
RRID:AB_2534069
This polyclonal secondary targets IgG (H+L)
View all literature mentionsCALCOCO2 antibody (antibody)
RRID:AB_1640255
This polyclonal targets CALCOCO2
View all literature mentionsAnti-Mono- and polyubiquitinylated conjugates Monoclonal Antibody, Unconjugated, Clone FK2 (antibody)
RRID:AB_2051891
This monoclonal targets Mono- and polyubiquitinylated conjugates
View all literature mentionsA-549 (cell line)
RRID:CVCL_0023
Cell line A-549 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsMcCoy (cell line)
RRID:CVCL_3742
Cell line McCoy is a Spontaneously immortalized cell line with a species of origin Mus musculus
View all literature mentions
