Poc1 bridges basal body inner junctions to promote triplet microtubule integrity and connections.
Basal bodies (BBs) are conserved eukaryotic structures that organize cilia. They are comprised of nine, cylindrically arranged, triplet microtubules (TMTs) connected to each other by inter-TMT linkages which stabilize the structure. Poc1 is a conserved protein important for BB structural integrity in the face of ciliary forces transmitted to BBs. To understand how Poc1 confers BB stability, we identified the precise position of Poc1 in the Tetrahymena BB and the effect of Poc1 loss on BB structure. Poc1 binds at the TMT inner junctions, stabilizing TMTs directly. From this location, Poc1 also stabilizes inter-TMT linkages throughout the BB, including the cartwheel pinhead and the inner scaffold. The full localization of the inner scaffold protein Fam161A requires Poc1. As ciliary forces are increased, Fam161A is reduced, indicative of a force-dependent molecular remodeling of the inner scaffold. Thus, while not essential for BB assembly, Poc1 promotes BB interconnections that establish an architecture competent to resist ciliary forces.
Pubmed ID: 38743010 RIS Download
Research resources used in this publication
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Antibodies used in this publication
- Living Colors® mCherry Monoclonal Antibody (RRID:AB_2307319)
- Anti-acetyl-alpha tubulin Antibody, clone 6-11B-1 (RRID:AB_2819178)
- tubulin (alpha-) antibody - Frankel, J. / Nelsen, E.M.; University of Iowa (RRID:AB_1157911)
- mCherry, rabbit polyclonal, Cat# RPCA-mCherry (RRID:AB_2571870)
- Purified Rabbit Anti-GFP, Anti-GFP (RRID:AB_10013661)
Associated grants
- Agency: NIGMS NIH HHS, United States
Id: R01 GM127571 - Agency: NIH HHS, United States
Id: R35GM140813 - Agency: NIGMS NIH HHS, United States
Id: R35 GM118099 - Agency: NIGMS NIH HHS, United States
Id: 5F32GM122239 - Agency: NIGMS NIH HHS, United States
Id: R35 GM140813 - Agency: NIGMS NIH HHS, United States
Id: F32 GM122239
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ImageJ (tool)
RRID:SCR_003070
Open source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
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A free, cross-platform set of image processing, modeling and display programs used for tomographic reconstruction and for 3D reconstruction of EM serial sections and optical sections. The package contains tools for assembling and aligning data within multiple types and sizes of image stacks, viewing 3-D data from any orientation, and modeling and display of the image files. IMOD 4.1.8 Is Now Available for Linux, Windows, and Mac OS X
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RRID:TSC_SD00701
Tetrahymena thermophila with name SB1969 from TSC.
View all literature mentionsLiving Colors® mCherry Monoclonal Antibody (antibody)
RRID:AB_2307319
This monoclonal targets mCherry
View all literature mentionsAnti-acetyl-alpha tubulin Antibody, clone 6-11B-1 (antibody)
RRID:AB_2819178
This monoclonal targets acetyl-alpha tubulin
View all literature mentionstubulin (alpha-) antibody - Frankel, J. / Nelsen, E.M.; University of Iowa (antibody)
RRID:AB_1157911
This monoclonal targets tubulin (alpha-)
View all literature mentionsmCherry, rabbit polyclonal, Cat# RPCA-mCherry (antibody)
RRID:AB_2571870
This polyclonal targets
View all literature mentionsPurified Rabbit Anti-GFP, Anti-GFP (antibody)
RRID:AB_10013661
This polyclonal targets Citrine, EGFP, GFP, YFP
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