Circadian-clock-controlled endocrine and cytokine signals regulate multipotential innate lymphoid cell progenitors in the bone marrow.
Innate lymphoid cells (ILCs), strategically positioned throughout the body, undergo population declines over time. A solution to counteract this problem is timely mobilization of multipotential progenitors from the bone marrow. It remains unknown what triggers the mobilization of bone marrow ILC progenitors (ILCPs). We report that ILCPs are regulated by the circadian clock to emigrate and generate mature ILCs in the periphery. We found that circadian-clock-defective ILCPs fail to normally emigrate and generate ILCs. We identified circadian-clock-controlled endocrine and cytokine cues that, respectively, regulate the retention and emigration of ILCPs at distinct times of each day. Activation of the stress-hormone-sensing glucocorticoid receptor upregulates CXCR4 on ILCPs for their retention in the bone marrow, while the interleukin-18 (IL-18) and RORα signals upregulate S1PR1 on ILCPs for their mobilization to the periphery. Our findings establish important roles of circadian signals for the homeostatic efflux of bone marrow ILCPs.
Pubmed ID: 38717905 RIS Download
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- APC anti-mouse IL-13 (RRID:AB_2910327)
- APC anti-mouse IL-10 (RRID:AB_315363)
- APC/Cyanine7 anti-mouse IL-17A (RRID:AB_2565781)
- PE anti-mouse IFN-gamma (RRID:AB_315402)
- PE anti-mouse IL-22 (RRID:AB_2124255)
- Rat Anti-Mouse Laminin alpha 1 Monoclonal antibody, Unconjugated, Clone al-4 (RRID:AB_2265518)
- Glucocorticoid Receptor (D6H2L) XP® Rabbit mAb (RRID:AB_2631286)
- PE/Cyanine7 anti-mouse PLZF (RRID:AB_2566165)
- Gata-3 Monoclonal Antibody (TWAJ), eFluor™ 660, eBioscience (RRID:AB_10596663)
- InVivoMab anti-mouse IL-18 (RRID:AB_2687719)
- APC anti-mouse CD49d (RRID:AB_2565777)
- PerCP anti-mouse TER-119/Erythroid Cells (RRID:AB_893635)
- PerCP anti-mouse NK-1.1 (RRID:AB_2132707)
- PerCP anti-mouse Ly-6G/Ly-6C (Gr-1) (RRID:AB_893557)
- BV421 Mouse Anti-Mouse RORγt Antibody (RRID:AB_2687545)
- PerCP anti-mouse/human CD45R/B220 (RRID:AB_893353)
- PerCP anti-mouse CD19 (RRID:AB_2072926)
- PerCP anti-mouse/human CD11b (RRID:AB_2129374)
- PerCP anti-mouse CD11c (RRID:AB_2129643)
- T-bet Monoclonal Antibody (eBio4B10 (4B10)), PE, eBioscience (RRID:AB_925761)
- PerCP anti-mouse CD4 (RRID:AB_893323)
- PerCP anti-mouse CD8a (RRID:AB_893423)
- PerCP anti-mouse CD3epsilon (RRID:AB_893317)
- Purified anti-mouse CD186 (CXCR6) (RRID:AB_2566544)
- Alexa Fluor(R) 488 anti-mouse/rat CD29 (RRID:AB_492829)
- Purified anti-mouse CD192 (CCR2) Recombinant (RRID:AB_2876563)
- FITC anti-mouse Ly-6C (RRID:AB_1186135)
- APC anti-mouse CD184 (CXCR4) (RRID:AB_2562785)
- Mouse EDG-1/S1P1 MAb (Clone 713412) (RRID:AB_10994183)
- APC anti-mouse CD28 (RRID:AB_312875)
- APC anti-mouse IL-23R (RRID:AB_2687346)
- APC/Cyanine7 anti-mouse CD335 (NKp46) (RRID:AB_2876479)
- APC anti-mouse CD117 (c-kit) (RRID:AB_2028407)
- APC anti-mouse CD135 (RRID:AB_2107050)
- PE anti-mouse CD159a (NKG2AB6) (RRID:AB_10965542)
- PE anti-mouse LPAM-1 (Integrin alpha4beta7) (RRID:AB_493267)
- Brilliant Violet 510(TM) anti-mouse/human KLRG1 (MAFA) (RRID:AB_2563800)
- Pacific Blue(TM) anti-mouse Ly-6A/E (Sca-1) (RRID:AB_493273)
- APC/Cyanine7 anti-mouse CD127 (IL-7Ralpha) (RRID:AB_2566161)
- APC anti-mouse CD279 (PD-1) (RRID:AB_2159183)
- PE anti-mouse CD122 (IL-2Rbeta) (RRID:AB_2125736)
- PE/Cyanine7 anti-mouse CD90.2 (Thy1.2) (RRID:AB_10643586)
- FITC anti-mouse CD45.1 (RRID:AB_313495)
- Brilliant Violet 510(TM) anti-mouse CD45.2 (RRID:AB_2650900)
- APC anti-mouse CD25 (RRID:AB_2280288)
Associated grants
- Agency: NCI NIH HHS, United States
Id: P30 CA046592 - Agency: NIAID NIH HHS, United States
Id: R01 AI121302 - Agency: NIAID NIH HHS, United States
Id: R56 AI173179 - Agency: NIAID NIH HHS, United States
Id: R01 AI173179 - Agency: NIAID NIH HHS, United States
Id: R21 AI148898
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Cell Signaling Technology (tool)
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THIS RESOURCE IS NO LONGER IN SERVICE. Documented on May 5,2022.Tool that predicts interactions between transcription factors and their regulated genes from binding motifs. Understanding vertebrate development requires unraveling the cis-regulatory architecture of gene regulation. PRISM provides accurate genome-wide computational predictions of transcription factor binding sites for the human and mouse genomes, and integrates the predictions with GREAT to provide functional biological context. Together, accurate computational binding site prediction and GREAT produce for each transcription factor: 1. putative binding sites, 2. putative target genes, 3. putative biological roles of the transcription factor, and 4. putative cis-regulatory elements through which the factor regulates each target in each functional role.
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Mus musculus with name C57BL/6J from IMSR.
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This monoclonal targets IL-17A
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RRID:AB_2124255
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View all literature mentionsRat Anti-Mouse Laminin alpha 1 Monoclonal antibody, Unconjugated, Clone al-4 (antibody)
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This monoclonal targets Mouse Laminin alpha 1
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RRID:AB_2631286
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This monoclonal targets Gata-3
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This monoclonal targets IL-18
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This monoclonal targets CD49d
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This monoclonal targets TER-119
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This monoclonal targets Ly-6G Ly-6C
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This monoclonal targets CD186
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This monoclonal targets CD29
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This recombinant monoclonal targets CD192
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This monoclonal targets CD184
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This monoclonal targets Mouse EDG-1/S1P1 MAb (Clone 713412)
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RRID:AB_312875
This monoclonal targets CD28
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This monoclonal targets CD117
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This monoclonal targets Ly-6A/E
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This monoclonal targets CD45.2
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This monoclonal targets CD25
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RRID:IMSR_JAX:006584
Mus musculus with name CByJ.SJL(B6)-Ptprca/J from IMSR.
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