CD80 on skin stem cells promotes local expansion of regulatory T cells upon injury to orchestrate repair within an inflammatory environment.
Following tissue damage, epithelial stem cells (SCs) are mobilized to enter the wound, where they confront harsh inflammatory environments that can impede their ability to repair the injury. Here, we investigated the mechanisms that protect skin SCs within this inflammatory environment. Characterization of gene expression profiles of hair follicle SCs (HFSCs) that migrated into the wound site revealed activation of an immune-modulatory program, including expression of CD80, major histocompatibility complex class II (MHCII), and CXC motif chemokine ligand 5 (CXCL5). Deletion of CD80 in HFSCs impaired re-epithelialization, reduced accumulation of peripherally generated Treg (pTreg) cells, and increased infiltration of neutrophils in wounded skin. Importantly, similar wound healing defects were also observed in mice lacking pTreg cells. Our findings suggest that upon skin injury, HFSCs establish a temporary protective network by promoting local expansion of Treg cells, thereby enabling re-epithelialization while still kindling inflammation outside this niche until the barrier is restored.
Pubmed ID: 38677291 RIS Download
Research resources used in this publication
- B6.Cg-Rag2tm1.1Cgn/J (RRID:IMSR_JAX:008449) (organism)
- B6.129S2-H2dlAb1-Ea/J (RRID:IMSR_JAX:003584) (organism)
- B6.129X1-H2-Ab1b-tm1Koni/J (RRID:IMSR_JAX:013181) (organism)
- STOCK Foxp3tm9(EGFP/cre/ERT2)Ayr/J (RRID:IMSR_JAX:016961) (organism)
-
B6.SJL-Ptprca Pepc
- B6.Cg-Tg(TcraTcrb)425Cbn/J (RRID:IMSR_JAX:004194) (organism)
- B6.129(Cg)-Foxp3tm3(Hbegf/GFP)Ayr/J (RRID:IMSR_JAX:016958) (organism)
- C57BL/6J (RRID:IMSR_JAX:000664) (organism)
- B6.129S4-Cd80tm1Shr/J (RRID:IMSR_JAX:003611) (organism)
- B6;129S6-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J (RRID:IMSR_JAX:007905) (organism)
Additional research tools detected in this publication
None foundAntibodies used in this publication
- PE anti-mouse CD45.1 (RRID:AB_313496)
- Rat Anti-Mouse Lix Monoclonal antibody, Unconjugated, Clone 61905 (RRID:AB_2086587)
- CD28 Monoclonal Antibody (37.51), Functional Grade, eBioscience (RRID:AB_468921)
- CD3e Monoclonal Antibody (145-2C11), eBioscience (RRID:AB_467049)
- Rhodamine Red-X-AffiniPure Donkey Anti-Rat IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Rb,Shp Sr Prot) (RRID:AB_2340675)
- Alexa Fluor 647-AffiniPure Donkey Anti-Chicken IgY (IgG) (H+L) (min X Bov,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rb,Rat,Shp Sr Prot) (RRID:AB_2340379)
- Alexa Fluor 647-AffiniPure Donkey Anti-Rabbit IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rat,Shp Sr Prot) (RRID:AB_2492288)
- Alexa Fluor 488-AffiniPure Donkey Anti-Goat IgG (H+L) (min X Ck,GP,Sy Hms,Hrs,Hu,Ms,Rb,Rat Sr Prot) (RRID:AB_2336933)
- Alexa Fluor 488 AffiniPure Donkey Anti-Chicken IgY (IgG) (H+L) (RRID:AB_2340375)
- Alexa Fluor 488-AffiniPure Donkey Anti-Rabbit IgG (H+L) (RRID:AB_2313584)
- Goat Anti-Mouse B7-1 / cd80 Polyclonal antibody, Unconjugated (RRID:AB_2075997)
- Purified anti-mouse Ly-6G (RRID:AB_1089180)
- Purified anti-mouse CD3 (RRID:AB_312659)
- Rat Anti-CD49e Monoclonal Antibody, Unconjugated, Clone 5H10-27 (RRID:AB_394779)
- Purified anti-Keratin 10 (RRID:AB_2616955)
- Purified anti-Keratin 14 (RRID:AB_2616962)
- GFP (RRID:AB_2313768)
- Biotin anti-mouse CD117 (c-kit) (RRID:AB_313213)
- Biotin anti-mouse CD31 (RRID:AB_312911)
- Alexa Fluor 700 Rat IgG2b, kappa Isotype Ctrl (RRID:AB_493783)
- Biotin anti-mouse CD45 (RRID:AB_312969)
- Biotin anti-mouse/human CD11b (RRID:AB_312787)
- APC Armenian Hamster IgG Isotype Ctrl Antibody (RRID:AB_2905474)
- FOXP3 Monoclonal Antibody (FJK-16s), APC, eBioscience (RRID:AB_469457)
- Brilliant Violet 711(TM) anti-mouse CD64 (FcgammaRI) (RRID:AB_2563846)
- PE anti-mouse Ly-6G (RRID:AB_1186099)
- PerCP/Cyanine5.5 anti-mouse Ly-6C (RRID:AB_1659242)
- Biotin anti-mouse CD140a (RRID:AB_2043974)
- FITC anti-mouse Ly-6C (RRID:AB_1186135)
- FITC anti-mouse IFN-gamma (RRID:AB_315400)
- PerCP/Cyanine5.5 anti-mouse IL-17A (RRID:AB_961384)
- PE anti-mouse IL-2 (RRID:AB_315302)
- Alexa Fluor(R) 700 anti-mouse I-A/I-E (RRID:AB_493727)
- PerCP/Cyanine5.5 anti-mouse Ki-67 (RRID:AB_2629531)
- PE anti-mouse TCR gamma/delta (RRID:AB_313832)
- Brilliant Violet 711(TM) anti-mouse TCR beta chain (RRID:AB_2629564)
- PE/Cyanine7 anti-mouse CD49e (RRID:AB_2734165)
- APC anti-mouse CD80 (RRID:AB_313135)
- Pacific Blue(TM) anti-mouse/human CD11b (RRID:AB_755986)
- APC/Cyanine7 anti-mouse CD45.2 (RRID:AB_830789)
- PE/Cyanine7 anti-mouse CD45.1 (RRID:AB_1134168)
- Brilliant Violet 711(TM) anti-mouse CD62L (RRID:AB_2564215)
- APC/Cyanine7 anti-mouse/human CD44 (RRID:AB_830785)
- FITC anti-mouse CD45 (RRID:AB_312973)
- PE anti-mouse CD45 (RRID:AB_312971)
- PE/Cyanine7 anti-mouse CD11c (RRID:AB_493568)
- APC/Cyanine7 anti-mouse CD45 (RRID:AB_312981)
- Alexa Fluor(R) 700 anti-mouse CD45 (RRID:AB_493715)
- APC/Cyanine7 anti-mouse CD8b (Ly-3) (RRID:AB_2563951)
- PE/Cyanine7 anti-mouse CD4 (RRID:AB_312707)
- PerCP/Cyanine5.5 anti-mouse/human CD11b (RRID:AB_893232)
- APC anti-mouse CD4 (RRID:AB_312697)
Associated grants
- Agency: NIAMS NIH HHS, United States
Id: R37 AR027883 - Agency: NIGMS NIH HHS, United States
Id: R35 GM150610 - Agency: NCI NIH HHS, United States
Id: R00 CA237859 - Agency: NCI NIH HHS, United States
Id: R01 CA285786 - Agency: NCI NIH HHS, United States
Id: P30 CA008748 - Agency: NIAID NIH HHS, United States
Id: T32 AI007090 - Agency: NIAMS NIH HHS, United States
Id: R01 AR050452 - Agency: NIAMS NIH HHS, United States
Id: R01 AR031737 - Agency: NIAMS NIH HHS, United States
Id: F31 AR073110
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
This is a list of tools and resources that we have found mentioned in this publication.
PE anti-mouse CD45.1 (antibody)
RRID:AB_313496
This monoclonal targets CD45.1
View all literature mentionsRat Anti-Mouse Lix Monoclonal antibody, Unconjugated, Clone 61905 (antibody)
RRID:AB_2086587
This monoclonal targets Mouse LIX
View all literature mentionsCD28 Monoclonal Antibody (37.51), Functional Grade, eBioscience (antibody)
RRID:AB_468921
This monoclonal targets CD28
View all literature mentionsCD3e Monoclonal Antibody (145-2C11), eBioscience (antibody)
RRID:AB_467049
This monoclonal targets CD3e
View all literature mentionsRhodamine Red-X-AffiniPure Donkey Anti-Rat IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Rb,Shp Sr Prot) (antibody)
RRID:AB_2340675
This unknown targets Rat IgG (H+L)
View all literature mentionsAlexa Fluor 647-AffiniPure Donkey Anti-Chicken IgY (IgG) (H+L) (min X Bov,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rb,Rat,Shp Sr Prot) (antibody)
RRID:AB_2340379
This unknown targets Chicken IgY (IgG) (H+L)
View all literature mentionsAlexa Fluor 647-AffiniPure Donkey Anti-Rabbit IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rat,Shp Sr Prot) (antibody)
RRID:AB_2492288
This polyclonal targets Rabbit IgG
View all literature mentionsAlexa Fluor 488-AffiniPure Donkey Anti-Goat IgG (H+L) (min X Ck,GP,Sy Hms,Hrs,Hu,Ms,Rb,Rat Sr Prot) (antibody)
RRID:AB_2336933
This polyclonal targets goat IgG
View all literature mentionsAlexa Fluor 488 AffiniPure Donkey Anti-Chicken IgY (IgG) (H+L) (antibody)
RRID:AB_2340375
This polyclonal targets IgY (IgG) (H+L)
View all literature mentionsAlexa Fluor 488-AffiniPure Donkey Anti-Rabbit IgG (H+L) (antibody)
RRID:AB_2313584
This polyclonal secondary targets IgG (H+L)
View all literature mentionsGoat Anti-Mouse B7-1 / cd80 Polyclonal antibody, Unconjugated (antibody)
RRID:AB_2075997
This polyclonal targets Mouse B7-1 / CD80
View all literature mentionsPurified anti-mouse Ly-6G (antibody)
RRID:AB_1089180
This monoclonal targets Ly-6G
View all literature mentionsPurified anti-mouse CD3 (antibody)
RRID:AB_312659
This monoclonal targets CD3
View all literature mentionsRat Anti-CD49e Monoclonal Antibody, Unconjugated, Clone 5H10-27 (antibody)
RRID:AB_394779
This monoclonal targets CD49e
View all literature mentionsPurified anti-Keratin 10 (antibody)
RRID:AB_2616955
This unknown targets Keratin 10
View all literature mentionsPurified anti-Keratin 14 (antibody)
RRID:AB_2616962
This unknown targets Keratin 14
View all literature mentionsBiotin anti-mouse CD117 (c-kit) (antibody)
RRID:AB_313213
This monoclonal targets CD117
View all literature mentionsBiotin anti-mouse CD31 (antibody)
RRID:AB_312911
This monoclonal targets CD31
View all literature mentionsAlexa Fluor 700 Rat IgG2b, kappa Isotype Ctrl (antibody)
RRID:AB_493783
This unknown targets Rat IgG2b kappa Isotype Control Alexa Fluor?? 700 Clone RTK4530
View all literature mentionsBiotin anti-mouse CD45 (antibody)
RRID:AB_312969
This monoclonal targets CD45
View all literature mentionsBiotin anti-mouse/human CD11b (antibody)
RRID:AB_312787
This monoclonal targets CD11b
View all literature mentionsAPC Armenian Hamster IgG Isotype Ctrl Antibody (antibody)
RRID:AB_2905474
This isotype control targets
View all literature mentionsFOXP3 Monoclonal Antibody (FJK-16s), APC, eBioscience (antibody)
RRID:AB_469457
This monoclonal targets FOXP3
View all literature mentionsBrilliant Violet 711(TM) anti-mouse CD64 (FcgammaRI) (antibody)
RRID:AB_2563846
This monoclonal targets CD64
View all literature mentionsPE anti-mouse Ly-6G (antibody)
RRID:AB_1186099
This monoclonal targets Ly-6G
View all literature mentionsPerCP/Cyanine5.5 anti-mouse Ly-6C (antibody)
RRID:AB_1659242
This monoclonal targets Ly-6C
View all literature mentionsBiotin anti-mouse CD140a (antibody)
RRID:AB_2043974
This monoclonal targets CD140a
View all literature mentionsFITC anti-mouse Ly-6C (antibody)
RRID:AB_1186135
This monoclonal targets Ly-6C
View all literature mentionsFITC anti-mouse IFN-gamma (antibody)
RRID:AB_315400
This monoclonal targets IFN-gamma
View all literature mentionsPerCP/Cyanine5.5 anti-mouse IL-17A (antibody)
RRID:AB_961384
This monoclonal targets IL-17A
View all literature mentionsPE anti-mouse IL-2 (antibody)
RRID:AB_315302
This monoclonal targets IL-2
View all literature mentionsAlexa Fluor(R) 700 anti-mouse I-A/I-E (antibody)
RRID:AB_493727
This monoclonal targets I-A/I-E
View all literature mentionsPerCP/Cyanine5.5 anti-mouse Ki-67 (antibody)
RRID:AB_2629531
This monoclonal targets Ki-67
View all literature mentionsPE anti-mouse TCR gamma/delta (antibody)
RRID:AB_313832
This monoclonal targets TCR gamma/delta
View all literature mentionsBrilliant Violet 711(TM) anti-mouse TCR beta chain (antibody)
RRID:AB_2629564
This monoclonal targets TCR beta chain
View all literature mentionsPE/Cyanine7 anti-mouse CD49e (antibody)
RRID:AB_2734165
This monoclonal targets CD49e
View all literature mentionsAPC anti-mouse CD80 (antibody)
RRID:AB_313135
This monoclonal targets CD80
View all literature mentionsPacific Blue(TM) anti-mouse/human CD11b (antibody)
RRID:AB_755986
This monoclonal targets CD11b
View all literature mentionsAPC/Cyanine7 anti-mouse CD45.2 (antibody)
RRID:AB_830789
This monoclonal targets CD45.2
View all literature mentionsPE/Cyanine7 anti-mouse CD45.1 (antibody)
RRID:AB_1134168
This monoclonal targets CD45.1
View all literature mentionsBrilliant Violet 711(TM) anti-mouse CD62L (antibody)
RRID:AB_2564215
This monoclonal targets CD62L
View all literature mentionsAPC/Cyanine7 anti-mouse/human CD44 (antibody)
RRID:AB_830785
This monoclonal targets CD44
View all literature mentionsFITC anti-mouse CD45 (antibody)
RRID:AB_312973
This monoclonal targets CD45
View all literature mentionsPE anti-mouse CD45 (antibody)
RRID:AB_312971
This monoclonal targets CD45
View all literature mentionsPE/Cyanine7 anti-mouse CD11c (antibody)
RRID:AB_493568
This monoclonal targets CD11c
View all literature mentionsAPC/Cyanine7 anti-mouse CD45 (antibody)
RRID:AB_312981
This monoclonal targets CD45
View all literature mentionsAlexa Fluor(R) 700 anti-mouse CD45 (antibody)
RRID:AB_493715
This monoclonal targets CD45
View all literature mentionsAPC/Cyanine7 anti-mouse CD8b (Ly-3) (antibody)
RRID:AB_2563951
This monoclonal targets CD8b
View all literature mentionsPE/Cyanine7 anti-mouse CD4 (antibody)
RRID:AB_312707
This monoclonal targets CD4
View all literature mentionsPerCP/Cyanine5.5 anti-mouse/human CD11b (antibody)
RRID:AB_893232
This monoclonal targets CD11b
View all literature mentionsAPC anti-mouse CD4 (antibody)
RRID:AB_312697
This monoclonal targets CD4
View all literature mentionsB6.Cg-Rag2tm1.1Cgn/J (organism)
RRID:IMSR_JAX:008449
Mus musculus with name B6.Cg-Rag2tm1.1Cgn/J from IMSR.
View all literature mentionsB6.129S2-H2dlAb1-Ea/J (organism)
RRID:IMSR_JAX:003584
Mus musculus with name B6.129S2-H2dlAb1-Ea/J from IMSR.
View all literature mentionsB6.129X1-H2-Ab1b-tm1Koni/J (organism)
RRID:IMSR_JAX:013181
Mus musculus with name B6.129X1-H2-Ab1b-tm1Koni/J from IMSR.
View all literature mentionsSTOCK Foxp3tm9(EGFP/cre/ERT2)Ayr/J (organism)
RRID:IMSR_JAX:016961
Mus musculus with name STOCK Foxp3tm9(EGFP/cre/ERT2)Ayr/J from IMSR.
View all literature mentions
B6.SJL-Ptprca Pepc/BoyJ (organism)
RRID:IMSR_JAX:002014
Mus musculus with name B6.SJL-Ptprca Pepc
B6.Cg-Tg(TcraTcrb)425Cbn/J (organism)
RRID:IMSR_JAX:004194
Mus musculus with name B6.Cg-Tg(TcraTcrb)425Cbn/J from IMSR.
View all literature mentionsB6.129(Cg)-Foxp3tm3(Hbegf/GFP)Ayr/J (organism)
RRID:IMSR_JAX:016958
Mus musculus with name B6.129(Cg)-Foxp3tm3(Hbegf/GFP)Ayr/J from IMSR.
View all literature mentionsC57BL/6J (organism)
RRID:IMSR_JAX:000664
Mus musculus with name C57BL/6J from IMSR.
View all literature mentionsB6.129S4-Cd80tm1Shr/J (organism)
RRID:IMSR_JAX:003611
Mus musculus with name B6.129S4-Cd80tm1Shr/J from IMSR.
View all literature mentionsB6;129S6-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J (organism)
RRID:IMSR_JAX:007905
Mus musculus with name B6;129S6-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J from IMSR.
View all literature mentions
