TBL38 atypical homogalacturonan-acetylesterase activity and cell wall microdomain localization in Arabidopsis seed mucilage secretory cells.
Plant cell walls constitute complex polysaccharidic/proteinaceous networks whose biosynthesis and dynamics implicate several cell compartments. The synthesis and remodeling of homogalacturonan pectins involve Golgi-localized methylation/acetylation and subsequent cell wall-localized demethylation/deacetylation. So far, TRICHOME BIREFRINGENCE-LIKE (TBL) family members have been described as Golgi-localized acetyltransferases targeting diverse hemicelluloses or pectins. Using seed mucilage secretory cells (MSCs) from Arabidopsis thaliana, we demonstrate the atypical localization of TBL38 restricted to a cell wall microdomain. A tbl38 mutant displays an intriguing homogalacturonan immunological phenotype in this cell wall microdomain and in an MSC surface-enriched abrasion powder. Mass spectrometry oligosaccharide profiling of this fraction reveals an increased homogalacturonan acetylation phenotype. Finally, TBL38 displays pectin acetylesterase activity in vitro. These results indicate that TBL38 is an atypical cell wall-localized TBL that displays a homogalacturonan acetylesterase activity rather than a Golgi-localized acetyltransferase activity as observed in previously studied TBLs. TBL38 function during seed development is discussed.
Pubmed ID: 38665206 RIS Download
Research resources used in this publication
- MZmine (RRID:SCR_012040) (software resource)
- Volocity 3D Image Analysis Software (RRID:SCR_002668) (software resource)
- R Project for Statistical Computing (RRID:SCR_001905) (software resource)
- AutoDock Vina (RRID:SCR_011958) (software resource)
- AutoDock (RRID:SCR_012746) (software resource)
- Microsoft Excel (RRID:SCR_016137) (software resource)
- ImageJ (RRID:SCR_003070) (software resource)
- BAR (RRID:SCR_006748) (data analysis service)
Additional research tools detected in this publication
- GenScript (RRID:SCR_002891)
- RIKEN BioResource Center (RRID:SCR_003250)
- Glyco3D (RRID:SCR_003797)
- NCBI Epigenomics (RRID:SCR_006151)
- Promega (RRID:SCR_006724)
- Thermo Fisher Scientific (RRID:SCR_008452)
- MP Biomedicals (RRID:SCR_013308)
- test (RRID:SCR_018743)
- University of Nottingham NASC Nottingham Arabidopsis Stock Centre Core Facility (RRID:SCR_019284)
Antibodies used in this publication
- Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in goat (RRID:AB_258103)
- Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (RRID:AB_2576217)
- Goat Anti-Mouse IgG (whole molecule) Polyclonal Antibody, Alkaline Phosphatase Conjugated (RRID:AB_258225)
- Anti-Rat IgG (whole molecule)-Alkaline Phosphatase antibody produced in goat (RRID:AB_258391)
- Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (RRID:AB_2534074)
- TagRFP Polyclonal Antibody (RRID:AB_10563941)
- Anti-homogalacturonan (RRID:AB_2734788)
- Anti-homogalacturonan (RRID:AB_2734789)
- Anti-Digoxigenin-AP, Fab fragments (RRID:AB_2734716)
Associated grants
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This is a list of tools and resources that we have found mentioned in this publication.
GenScript (tool)
RRID:SCR_002891
Commercial organization which provides life science services and products to researchers. They specialize in gene synthesis, peptide, protein, antibody and preclinical drug development service.
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View all literature mentionsNCBI Epigenomics (tool)
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THIS RESOURCE IS NO LONGER IN SERVICE, documented on January 19, 2022.
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RRID:SCR_019284
National and international arabidopsis germplasm resource.Stores over million genotypes in physical stocks servicing worldwide Arabidopsis community in more than 50 countries. Stocks are provided under identical cost recovery conditions to academic and commercial researchers. Non-transgenic stocks are sent gratis to K-17 institutions and in special cases. Provides seed and information resources to International Arabidopsis Genome Programme and research community. Maintains accessions of Arabidopsis thaliana (and small number of other related species), including Characterized lines, Mapping populations, and Insertion lines. You may browse or search catalog.
View all literature mentionsAnti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in goat (antibody)
RRID:AB_258103
This polyclonal targets Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in goat
View all literature mentionsGoat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (antibody)
RRID:AB_2576217
This polyclonal secondary targets IgG (H+L)
View all literature mentionsGoat Anti-Mouse IgG (whole molecule) Polyclonal Antibody, Alkaline Phosphatase Conjugated (antibody)
RRID:AB_258225
This unknown targets Mouse IgG, whole molecule
View all literature mentionsAnti-Rat IgG (whole molecule)-Alkaline Phosphatase antibody produced in goat (antibody)
RRID:AB_258391
This unknown targets Rat IgG, whole molecule
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RRID:AB_2534074
This polyclonal secondary targets IgG (H+L)
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RRID:SCR_002668
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RRID:SCR_001905
Software environment and programming language for statistical computing and graphics. R is integrated suite of software facilities for data manipulation, calculation and graphical display. Can be extended via packages. Some packages are supplied with the R distribution and more are available through CRAN family.It compiles and runs on wide variety of UNIX platforms, Windows and MacOS.
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Software application with data analysis tools and spreadsheet templates to track and visualize data. It is used to manage and process data.
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RRID:SCR_003070
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