Hippocampus-to-amygdala pathway drives the separation of remote memories of related events.
The mammalian brain can store and retrieve memories of related events as distinct memories and remember common features of those experiences. How it computes this function remains elusive. Here, we show in rats that recent memories of two closely timed auditory fear events share overlapping neuronal ensembles in the basolateral amygdala (BLA) and are functionally linked. However, remote memories have reduced neuronal overlap and are functionally independent. The activity of parvalbumin (PV)-expressing neurons in the BLA plays a crucial role in forming separate remote memories. Chemogenetic blockade of PV preserves individual remote memories but prevents their segregation, resulting in reciprocal associations. The hippocampus drives this process through specific excitatory connections with BLA GABAergic interneurons. These findings provide insights into the neuronal mechanisms that minimize the overlap between distinct remote memories and enable the retrieval of related memories separately.
Pubmed ID: 38656872 RIS Download
Research resources used in this publication
- pAAV-hSyn-DIO-mCherry (RRID:Addgene_50459) (plasmid)
- pENN.AAV.hSyn.Cre.WPRE.hGH (RRID:Addgene_105553) (plasmid)
- pAAV-hSyn-mCherry (RRID:Addgene_114472) (plasmid)
- pAAV-hSyn-DIO-hM4D(Gi)-mCherry (RRID:Addgene_44362) (plasmid)
- pAAV-hSyn-hM4D(Gi)-mCherry (RRID:Addgene_50475) (plasmid)
- Goat anti-Rabbit IgG (Heavy Chain), Superclonal Recombinant Secondary Antibody, Alexa Fluor™ 488 (RRID:AB_2536097) (antibody)
- Goat anti-Rabbit IgG (Heavy Chain), Superclonal Recombinant Secondary Antibody, Alexa Fluor™ 488 (RRID:AB_2536097) (antibody)
- Cy3-AffiniPure Goat Anti-Guinea Pig IgG (H+L) (RRID:AB_2337423) (antibody)
- Alexa Fluor 488-AffiniPure Goat Anti-Guinea Pig IgG (H+L) (RRID:AB_2337438) (antibody)
- Alexa Fluor 488-AffiniPure Goat Anti-Guinea Pig IgG (H+L) (RRID:AB_2337438) (antibody)
- Cy3-AffiniPure Goat Anti-Guinea Pig IgG (H+L) (RRID:AB_2337423) (antibody)
- Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Cyanine3 (RRID:AB_2534031) (antibody)
- Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Cyanine5 (RRID:AB_10562712) (antibody)
- Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Cyanine3 (RRID:AB_10563288) (antibody)
- Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Cyanine3 (RRID:AB_10563288) (antibody)
- Goat anti-Chicken IgY (H+L) Secondary Antibody, Alexa Fluor™ 488 (RRID:AB_2534096) (antibody)
- Goat anti-Chicken IgY (H+L) Secondary Antibody, Alexa Fluor™ 488 (RRID:AB_2534096) (antibody)
- Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Cyanine3 (RRID:AB_2534031) (antibody)
- Rabbit Anti-GAD65 + GAD67 Polyclonal Antibody, Unconjugated (RRID:AB_880149) (antibody)
- Rabbit Anti-GAD65 + GAD67 Polyclonal Antibody, Unconjugated (RRID:AB_880149) (antibody)
- Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Cyanine5 (RRID:AB_10562712) (antibody)
- Anti-RFP (RRID:AB_2737051) (antibody)
- Anti-RFP (RRID:AB_2737051) (antibody)
- Anti-Parvalbumin (RRID:AB_2156476) (antibody)
- Monoclonal Anti-Parvalbumin antibody produced in mouse (RRID:AB_477329) (antibody)
- c-Fos Antibody (RRID:AB_2106617) (antibody)
- GFP Polyclonal Antibody (RRID:AB_2534023) (antibody)
- Anti-c-Fos (RRID:AB_2864765) (antibody)
- Anti-Beta-Gal (Beta-Galactosidase, LacZ) Antibody (RRID:AB_2313508) (antibody)
- Mouse Anti-CaM Kinase II, alpha subunit Monoclonal antibody, Unconjugated (RRID:AB_309787) (antibody)
Additional research tools detected in this publication
None foundAntibodies used in this publication
- Goat anti-Rabbit IgG (Heavy Chain), Superclonal Recombinant Secondary Antibody, Alexa Fluor™ 488 (RRID:AB_2536097)
- Cy3-AffiniPure Goat Anti-Guinea Pig IgG (H+L) (RRID:AB_2337423)
- Alexa Fluor 488-AffiniPure Goat Anti-Guinea Pig IgG (H+L) (RRID:AB_2337438)
- Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Cyanine3 (RRID:AB_2534031)
- Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Cyanine5 (RRID:AB_10562712)
- Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Cyanine3 (RRID:AB_10563288)
- Goat anti-Chicken IgY (H+L) Secondary Antibody, Alexa Fluor™ 488 (RRID:AB_2534096)
- Rabbit Anti-GAD65 + GAD67 Polyclonal Antibody, Unconjugated (RRID:AB_880149)
- Anti-RFP (RRID:AB_2737051)
- Anti-Parvalbumin (RRID:AB_2156476)
- Anti-c-Fos (RRID:AB_2864765)
- Monoclonal Anti-Parvalbumin antibody produced in mouse (RRID:AB_477329)
- c-Fos Antibody (RRID:AB_2106617)
- GFP Polyclonal Antibody (RRID:AB_2534023)
- Anti-Beta-Gal (Beta-Galactosidase, LacZ) Antibody (RRID:AB_2313508)
- Mouse Anti-CaM Kinase II, alpha subunit Monoclonal antibody, Unconjugated (RRID:AB_309787)
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This is a list of tools and resources that we have found mentioned in this publication.
Goat anti-Rabbit IgG (Heavy Chain), Superclonal Recombinant Secondary Antibody, Alexa Fluor™ 488 (antibody)
RRID:AB_2536097
This recombinant polyclonal secondary targets IgG (Heavy Chain)
View all literature mentionsCy3-AffiniPure Goat Anti-Guinea Pig IgG (H+L) (antibody)
RRID:AB_2337423
This unknown targets Guinea Pig IgG (H+L)
View all literature mentionsAlexa Fluor 488-AffiniPure Goat Anti-Guinea Pig IgG (H+L) (antibody)
RRID:AB_2337438
This unknown targets Guinea Pig IgG (H+L)
View all literature mentionsGoat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Cyanine3 (antibody)
RRID:AB_2534031
This polyclonal secondary targets IgG (H+L)
View all literature mentionsGoat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Cyanine5 (antibody)
RRID:AB_10562712
This polyclonal secondary targets IgG (H+L)
View all literature mentionsGoat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Cyanine3 (antibody)
RRID:AB_10563288
This polyclonal secondary targets Rabbit IgG (H+L)
View all literature mentionsGoat anti-Chicken IgY (H+L) Secondary Antibody, Alexa Fluor™ 488 (antibody)
RRID:AB_2534096
This polyclonal secondary targets IgY (H+L)
View all literature mentionsRabbit Anti-GAD65 + GAD67 Polyclonal Antibody, Unconjugated (antibody)
RRID:AB_880149
This polyclonal targets GAD65 + GAD67
View all literature mentionsAnti-RFP (antibody)
RRID:AB_2737051
This polyclonal targets mRFP, mCherry, mOrgange2, dsRed, tdTomato, mScarlet
View all literature mentionsAnti-Parvalbumin (antibody)
RRID:AB_2156476
This polyclonal targets Parvalbumin
View all literature mentionsMonoclonal Anti-Parvalbumin antibody produced in mouse (antibody)
RRID:AB_477329
This monoclonal targets Parvalbumin
View all literature mentionsGFP Polyclonal Antibody (antibody)
RRID:AB_2534023
This unknown targets GFP
View all literature mentionsAnti-Beta-Gal (Beta-Galactosidase, LacZ) Antibody (antibody)
RRID:AB_2313508
This polyclonal targets BGL-1010
View all literature mentionsMouse Anti-CaM Kinase II, alpha subunit Monoclonal antibody, Unconjugated (antibody)
RRID:AB_309787
This monoclonal targets CaM Kinase II, alpha subunit
View all literature mentionsGoat anti-Rabbit IgG (Heavy Chain), Superclonal Recombinant Secondary Antibody, Alexa Fluor™ 488 (antibody)
RRID:AB_2536097
This recombinant polyclonal secondary targets IgG (Heavy Chain)
View all literature mentionsGoat anti-Rabbit IgG (Heavy Chain), Superclonal Recombinant Secondary Antibody, Alexa Fluor™ 488 (antibody)
RRID:AB_2536097
This recombinant polyclonal secondary targets IgG (Heavy Chain)
View all literature mentionsCy3-AffiniPure Goat Anti-Guinea Pig IgG (H+L) (antibody)
RRID:AB_2337423
This unknown targets Guinea Pig IgG (H+L)
View all literature mentionsAlexa Fluor 488-AffiniPure Goat Anti-Guinea Pig IgG (H+L) (antibody)
RRID:AB_2337438
This unknown targets Guinea Pig IgG (H+L)
View all literature mentionsAlexa Fluor 488-AffiniPure Goat Anti-Guinea Pig IgG (H+L) (antibody)
RRID:AB_2337438
This unknown targets Guinea Pig IgG (H+L)
View all literature mentionsCy3-AffiniPure Goat Anti-Guinea Pig IgG (H+L) (antibody)
RRID:AB_2337423
This unknown targets Guinea Pig IgG (H+L)
View all literature mentionsGoat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Cyanine3 (antibody)
RRID:AB_2534031
This polyclonal secondary targets IgG (H+L)
View all literature mentionsGoat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Cyanine5 (antibody)
RRID:AB_10562712
This polyclonal secondary targets IgG (H+L)
View all literature mentionsGoat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Cyanine3 (antibody)
RRID:AB_10563288
This polyclonal secondary targets Rabbit IgG (H+L)
View all literature mentionsGoat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Cyanine3 (antibody)
RRID:AB_10563288
This polyclonal secondary targets Rabbit IgG (H+L)
View all literature mentionsGoat anti-Chicken IgY (H+L) Secondary Antibody, Alexa Fluor™ 488 (antibody)
RRID:AB_2534096
This polyclonal secondary targets IgY (H+L)
View all literature mentionsGoat anti-Chicken IgY (H+L) Secondary Antibody, Alexa Fluor™ 488 (antibody)
RRID:AB_2534096
This polyclonal secondary targets IgY (H+L)
View all literature mentionsGoat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Cyanine3 (antibody)
RRID:AB_2534031
This polyclonal secondary targets IgG (H+L)
View all literature mentionsRabbit Anti-GAD65 + GAD67 Polyclonal Antibody, Unconjugated (antibody)
RRID:AB_880149
This polyclonal targets GAD65 + GAD67
View all literature mentionsRabbit Anti-GAD65 + GAD67 Polyclonal Antibody, Unconjugated (antibody)
RRID:AB_880149
This polyclonal targets GAD65 + GAD67
View all literature mentionsGoat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Cyanine5 (antibody)
RRID:AB_10562712
This polyclonal secondary targets IgG (H+L)
View all literature mentionsAnti-RFP (antibody)
RRID:AB_2737051
This polyclonal targets mRFP, mCherry, mOrgange2, dsRed, tdTomato, mScarlet
View all literature mentionsAnti-RFP (antibody)
RRID:AB_2737051
This polyclonal targets mRFP, mCherry, mOrgange2, dsRed, tdTomato, mScarlet
View all literature mentionsAnti-Parvalbumin (antibody)
RRID:AB_2156476
This polyclonal targets Parvalbumin
View all literature mentionsMonoclonal Anti-Parvalbumin antibody produced in mouse (antibody)
RRID:AB_477329
This monoclonal targets Parvalbumin
View all literature mentionsGFP Polyclonal Antibody (antibody)
RRID:AB_2534023
This unknown targets GFP
View all literature mentionsAnti-Beta-Gal (Beta-Galactosidase, LacZ) Antibody (antibody)
RRID:AB_2313508
This polyclonal targets BGL-1010
View all literature mentionsMouse Anti-CaM Kinase II, alpha subunit Monoclonal antibody, Unconjugated (antibody)
RRID:AB_309787
This monoclonal targets CaM Kinase II, alpha subunit
View all literature mentions
