ATCC (tool)

RRID:SCR_001672

Global nonprofit biological resource center (BRC) and research organization that provides biological products, technical services and educational programs to private industry, government and academic organizations. Its mission is to acquire, authenticate, preserve, develop and distribute biological materials, information, technology, intellectual property and standards for the advancement and application of scientific knowledge. The primary purpose of ATCC is to use its resources and experience as a BRC to become the world leader in standard biological reference materials management, intellectual property resource management and translational research as applied to biomaterial development, standardization and certification. ATCC characterizes cell lines, bacteria, viruses, fungi and protozoa, as well as develops and evaluates assays and techniques for validating research resources and preserving and distributing biological materials to the public and private sector research communities.

Addgene (tool)

RRID:SCR_002037

Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.

Cell Signaling Technology (tool)

RRID:SCR_002071

Privately held company that develops and produces antibodies, ELISA kits, ChIP kits, proteomic kits, and other related reagents used to study cell signaling pathways that impact human health.

Thermo Fisher Scientific (tool)

RRID:SCR_008452

Commercial vendor and service provider of laboratory reagents and antibodies. Supplier of scientific instrumentation, reagents and consumables, and software services.

Millipore (tool)

RRID:SCR_008983

An Antibody supplier

Proteintech Group (tool)

RRID:SCR_008986

Proteintech Europe Ltd is an ISO 9001:2008 certified company

Santa Cruz Biotechnology (tool)

RRID:SCR_008987

An Antibody supplier

Sigma-Aldrich (tool)

RRID:SCR_008988

American chemical, life science and biotechnology company owned by Merck KGaA. Merger of Sigma Chemical Company and Aldrich Chemical Company. Provides organic and inorganic chemicals, building blocks, reagents, advanced materials and stable isotopes for chemical synthesis, medicinal chemistry and materials science, antibiotics, buffers, carbohydrates, enzymes, forensic tools, hematology and histology, nucleotides, proteins, peptides, amino acids and their derivatives.

BD Biosciences (tool)

RRID:SCR_013311

An Antibody supplier

Microsoft Excel (tool)

RRID:SCR_016137

Software application with data analysis tools and spreadsheet templates to track and visualize data. It is used to manage and process data.

mouse-IgG-control-human (antibody)

RRID:AB_737182

This unknown targets mouse-IgG-control

Phospho-Tuberin/TSC2 (Ser1387) Antibody (antibody)

RRID:AB_10698883

This polyclonal targets Phospho-Tuberin/TSC2 (Ser1387)

Protein A/G PLUS-Agarose (antibody)

RRID:AB_10201400

This unknown targets Protein A/G PLUS-Agarose

Rabbit Normal igg Control antibody, Unconjugated (antibody)

RRID:AB_145841

This unknown targets Rabbit Normal igg Control antibody

5-Bromo-2′-deoxy-uridine Labeling and Detection Kit I (antibody)

RRID:AB_2814711

This monoclonal targets BrdU

Goat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor 488 Conjugated (antibody)

RRID:AB_143165

This polyclonal targets IgG (H+L)

Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 594 (antibody)

RRID:AB_2534073

This polyclonal secondary targets IgG (H+L)

Anti-mouse IgG, HRP-linked Antibody (antibody)

RRID:AB_330924

This polyclonal targets IgG

Anti-rabbit IgG, HRP-linked Antibody (antibody)

RRID:AB_2099233

This polyclonal secondary targets IgG

Histone H3ac (pan-acetyl) antibody (antibody)

RRID:AB_2687871

This polyclonal targets Histone H3ac (pan-acetyl)

Histone H4ac (pan-acetyl) antibody (pAb) (antibody)

RRID:AB_2793201

This unknown targets Histone H4ac (pan-acetyl)

Caspase-3 (antibody)

RRID:AB_397713

This monoclonal targets Caspase-3

vinculin Antibody (7F9) (antibody)

RRID:AB_1131294

This monoclonal targets vinculin

p27 (N-20) (antibody)

RRID:AB_632131

This polyclonal targets p27 (N-20)

EP300-human (antibody)

RRID:AB_2231120

This unknown targets EP300

p21 (C-19) (antibody)

RRID:AB_632126

This unknown targets Raised against a peptide mapping at the carboxy terminus of human p21

cyclin B1 (H-433) (antibody)

RRID:AB_2072134

This unknown targets epitope corresponding to amino acids 1-433 representing full length cyclin B1 of human origin

cyclin A (H-432) (antibody)

RRID:AB_631329

This polyclonal targets CCNA2

Rabbit Anti-Human Cyclin E (C-19) Polyclonal, Unconjugated (antibody)

RRID:AB_631346

This polyclonal targets Human CCNE1

cyclin D1 (H-295) (antibody)

RRID:AB_2070433

This polyclonal targets CCND1

Cleaved Caspase-3 (Asp175) Antibody (antibody)

RRID:AB_2341188

This polyclonal targets Cleaved Caspase-3 (Asp175)

Beclin-1 (D40C5) Rabbit mAb (antibody)

RRID:AB_1903911

This monoclonal targets Beclin-1

PARP Antibody (antibody)

RRID:AB_2160739

This polyclonal targets PARP

LC3A/B (D3U4C) XP® Rabbit mAb (antibody)

RRID:AB_2617131

This monoclonal targets LC3A/B

SQSTM1/p62 Antibody (antibody)

RRID:AB_10624872

This polyclonal targets SQSTM1/p62

Phospho-SMAD2 (Ser465/Ser467) (E8F3R) Rabbit mAb (antibody)

RRID:AB_2798798

This monoclonal targets SMAD2

c-Jun (60A8) Rabbit mAb (antibody)

RRID:AB_2130165

This monoclonal targets JUND

Phospho-c-Jun (Ser63) (54B3) Rabbit mAb (antibody)

RRID:AB_490908

This monoclonal targets Phospho-c-Jun (Ser63) (54B3) Rabbit mAb

Akt Antibody (antibody)

RRID:AB_329827

This polyclonal targets Akt

Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (antibody)

RRID:AB_2315049

This monoclonal targets Phospho-Akt (Ser473)

p44/42 MAPK (Erk1/2) Antibody (antibody)

RRID:AB_330744

This polyclonal targets p44/42 MAPK (Erk1/2)

p44/42 MAP kinase (phosphorylated Erk1/2) (antibody)

RRID:AB_331646

This polyclonal targets Erk1/2

Rabbit Anti-p70 S6 Kinase Monoclonal Antibody, Unconjugated, Clone 49D7 (antibody)

RRID:AB_390722

This monoclonal targets p70 S6 Kinase

Smad3 (C67H9) Rabbit mAb (antibody)

RRID:AB_2193182

This monoclonal targets Smad3

Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb (antibody)

RRID:AB_2269803

This monoclonal targets p70 S6 Kinase, phospho (Thr389)

Tuberin/TSC2 (D93F12) XP Rabbit mAb (antibody)

RRID:AB_10547134

This monoclonal targets TSC2

Acetyl-CoA Carboxylase Antibody (antibody)

RRID:AB_2219400

This polyclonal targets Acetyl CoA Carboxylase

Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb (antibody)

RRID:AB_2193207

This monoclonal targets Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb

Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody (antibody)

RRID:AB_330337

This polyclonal targets Phospho-Acetyl-CoA Carboxylase (Ser79)

Anti-AMPK-alpha Antibody, Unconjugated (antibody)

RRID:AB_330331

This polyclonal targets AMPK-alpha

Phospho-AMPKα (Thr172) Antibody (antibody)

RRID:AB_330330

This polyclonal targets Phospho-AMPKα (Thr172)

anti-BRD4 (E2A7X) Rabbit Antibody (antibody)

RRID:AB_2687578

This monoclonal targets BRD4

Smad2 (D43B4) XP Rabbit mAb (antibody)

RRID:AB_10626777

This monoclonal targets Smad2 (D43B4) XP Rabbit mAb

Anti-Histone H3 Antibody, Unconjugated (antibody)

RRID:AB_331563

This polyclonal targets Histone H3

Acetyl-Histone H3 (Lys27) Antibody (antibody)

RRID:AB_10545273

This polyclonal targets Acetyl-Histone H3 (Lys27)

SPARC Antibody (antibody)

RRID:AB_10692794

This polyclonal targets SPARC

V5 Tag Monoclonal Antibody (E10/V4RR) (antibody)

RRID:AB_10977225

This monoclonal targets V5 Tag

Anti-Histone Deacetylase 10 (HDAC10) antibody produced in rabbit (antibody)

RRID:AB_261940

This unknown targets Histone Deacetylase 10 (HDAC10)

Beta Actin antibody (antibody)

RRID:AB_2687938

This monoclonal targets Beta Actin

Acetylated Tubulin (antibody)

RRID:AB_609894

This monoclonal targets derived from the hybridoma 6-11B-1 produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with acetylated tubulin from the outer arm of Strongylocentrotus purpuratus (sea urchin).

tubulin alpha (antibody)

RRID:AB_2315509

This unknown targets

Monoclonal ANTI-FLAG® M2 antibody produced in mouse (antibody)

RRID:AB_262044

This monoclonal targets FLAG

A-375 (cell line)

RRID:CVCL_0132

Cell line A-375 is a Cancer cell line with a species of origin Homo sapiens (Human)

NCI-H1299 (cell line)

RRID:CVCL_0060

Cell line NCI-H1299 is a Cancer cell line with a species of origin Homo sapiens (Human)

WM793 (cell line)

RRID:CVCL_8787

Cell line WM793 is a Cancer cell line with a species of origin Homo sapiens (Human)

WM983A (cell line)

RRID:CVCL_6808

Cell line WM983A is a Cancer cell line with a species of origin Homo sapiens (Human)

SK-MEL-5 (cell line)

RRID:CVCL_0527

Cell line SK-MEL-5 is a Cancer cell line with a species of origin Homo sapiens (Human)

GraphPad Prism (software resource)

RRID:SCR_002798

Statistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.

pcDNA-dCas9-p300 Core (D1399Y) (plasmid)

RRID:Addgene_61358

ImageJ (software resource)

RRID:SCR_003070

Open source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.

NOD.Cg-Prkdc^scid Il2rg/SzJ (organism)

RRID:IMSR_JAX:005557

Mus musculus with name NOD.Cg-Prkdc^scid Il2rg/SzJ from IMSR.

lenti-sgRNA blast (plasmid)

RRID:Addgene_104993

pLenti CMV Neo DEST (705-1) (plasmid)

RRID:Addgene_17392

pcDNA-dCas9-p300 Core (plasmid)

RRID:Addgene_61357

pLKO.1 GFP shRNA (plasmid)

RRID:Addgene_30323

lentiCRISPR v2 (plasmid)

RRID:Addgene_52961

SK-MEL-28 (cell line)

RRID:CVCL_0526

Cell line SK-MEL-28 is a Cancer cell line with a species of origin Homo sapiens (Human)

SK-MEL-2 (cell line)

RRID:CVCL_0069

Cell line SK-MEL-2 is a Cancer cell line with a species of origin Homo sapiens (Human)

HEK293T (cell line)

RRID:CVCL_0063

Cell line HEK293T is a Transformed cell line with a species of origin Homo sapiens (Human)

A-549 (cell line)

RRID:CVCL_0023

Cell line A-549 is a Cancer cell line with a species of origin Homo sapiens (Human)

1205Lu (cell line)

RRID:CVCL_5239

Cell line 1205Lu is a Cancer cell line with a species of origin Homo sapiens (Human)

UACC-257 (cell line)

RRID:CVCL_1779

Cell line UACC-257 is a Cancer cell line with a species of origin Homo sapiens (Human)